Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-COX7B antibody [EPR9327(B)] (ab137094)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9327(B)] to COX7B
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-COX7B antibody [EPR9327(B)]
    See all COX7B primary antibodies

  • Description

    Rabbit monoclonal [EPR9327(B)] to COX7B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human COX7B aa 50-150. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa and MCF-7 cells; Fetal heart tissue lysate. IHC-P: Human colon and kidney tissues. ICC/IF: HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-COX7B antibody [EPR9327(B)] (ab137094)
    Western blot - Anti-COX7B antibody [EPR9327(B)] (ab137094)
    All lanes : Anti-COX7B antibody [EPR9327(B)] (ab137094) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : COX7B knockout HeLa cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 9 kDa
    Observed band size: 13 kDa
    why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab137094 observed at 13 kDa. Red - loading control ab8245 observed at 36 kDa.

    ab137094 Anti-COX7B antibody [EPR9327(B)] was shown to specifically react with COX7B in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265549 (knockout cell lysate ab257897) was used. Wild-type and COX7B knockout samples were subjected to SDS-PAGE. ab137094 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-COX7B antibody [EPR9327(B)] (ab137094)
    Immunocytochemistry/ Immunofluorescence - Anti-COX7B antibody [EPR9327(B)] (ab137094)
    Immunofluorescent staining of HepG2 cells labelling COX7B with ab137094 at 1/100 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (ab137094)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (ab137094)

    Immunohistochemical analysis using ab137094 showing positive staining in Thyroid gland papillary carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (ab137094)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (ab137094)

    Immunohistochemical analysis using ab137094 showing positive staining in Heart tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (ab137094)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (ab137094)

    Immunohistochemical analysis using ab137094 showing positive staining in Lung adenocarcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (ab137094)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (ab137094)

    Immunohistochemical analysis using ab137094 showing positive staining in Urinary bladder transitional carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (ab137094)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (ab137094)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling COX7B with ab137094 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (ab137094)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (ab137094)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling COX7B with ab137094 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-COX7B antibody [EPR9327(B)] (ab137094)
    Western blot - Anti-COX7B antibody [EPR9327(B)] (ab137094)
    Anti-COX7B antibody [EPR9327(B)] (ab137094) at 1/1000 dilution + Fetal heart lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 9 kDa

  • Anti-COX7B antibody [EPR9327(B)] (ab137094)
    Anti-COX7B antibody [EPR9327(B)] (ab137094)

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