Anti-BCKDHA antibody (ab138460)
Key features and details
- Rabbit polyclonal to BCKDHA
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BCKDHA antibody
See all BCKDHA primary antibodies -
Description
Rabbit polyclonal to BCKDHA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between amino acids 395 and 445 of Human BCKDHA (NP_000700.1).
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Positive control
- HeLa, 293T and Jurkat whole cell lysate (ab7899).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - Anti-BCKDHA antibody (ab138460)All lanes : Anti-BCKDHA antibody (ab138460) at 1/2000 dilution
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : 293T cell lysate at 50 µg
Lane 4 : Jurkat cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-BCKDHA antibody (ab138460)Immunoprecipitation detection of BCKDHA in HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane) using ab138460 at 6µg/mg lysate. Subsequent Western blot detection step was performed using ab138460 at 1µg/ml. Chemiluminescence with an exposure time of 30 seconds.
