Anti-POLG antibody (ab97661)
Key features and details
- Rabbit polyclonal to POLG
- Suitable for: ICC/IF, IP, IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-POLG antibody
See all POLG primary antibodies -
Description
Rabbit polyclonal to POLG -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Human POLG aa 779-1201.
Database link: NP_002684 -
Positive control
- WB: MCF7, MDA-MB-231 cell and rat testis tissue lysate; IHC-P: Human skeletal muscle tissue; IF: HeLa cells; IP: MCF7 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-POLG antibody (ab97661)All lanes : Anti-POLG antibody (ab97661) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 140 kDa5% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLG antibody (ab97661)Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue staining DNA polymerase gamma with ab97661 at 10 μg/ml.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min. -
Immunocytochemistry/ Immunofluorescence - Anti-POLG antibody (ab97661)Immunofluorescence analysis of methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
labeling DNA polymerase gamma with ab97661 at 1/200 dilution. Costained with Hoechst 33342. -
Immunoprecipitation - Anti-POLG antibody (ab97661)Immunoprecipitation of DNA polymerase gamma protein from MCF7 (human breast adenocarcinoma cell line) whole cell lysate using 5 μg ab97661.
IP Sample:
1- MCF7 cells
2- Control IgG
3- ab97661
Western blot analysis was performed using ab97661. followed by anti-Rabbit IgG antibody. -
Western blot - Anti-POLG antibody (ab97661)Anti-POLG antibody (ab97661) at 1/1000 dilution + Rat testis tissue lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 140 kDa5% SDS-PAGE
