Anti-DNA Polymerase lambda/Polk antibody (ab224823)
Key features and details
- Rabbit polyclonal to DNA Polymerase lambda/Polk
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-DNA Polymerase lambda/Polk antibody
See all DNA Polymerase lambda/Polk primary antibodies -
Description
Rabbit polyclonal to DNA Polymerase lambda/Polk -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide within Human DNA Polymerase lambda/Polk aa 150-200. The exact sequence is proprietary. (NP_037406.1).
Database link: Q9UGP5 -
Positive control
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysates.
-
General notes
This product was previously labelled as DNA Polymerase lambda
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab224823 was affinity purified using an epitope specific to DNA Polymerase lambda/Polk immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-DNA Polymerase lambda/Polk antibody (ab224823)All lanes : Anti-DNA Polymerase lambda/Polk antibody (ab224823) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 63 kDa
Exposure time: 3 minutesLystates prepared using NETN lysis buffer.
-
Immunoprecipitation - Anti-DNA Polymerase lambda/Polk antibody (ab224823)DNA Polymerase lambda/Polk was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer) with ab224823 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224823 at 1 µg/ml.
Lane 1: ab224823 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
