Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] (ab172974)
![Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] (ab172974)](https://www.abcam.com/ps/products/172/ab172974/Images/ab172974-383526-anti-dna-polymerase-lambdapolk-antibody-epr75192-western-blot-hela-a549.jpg "Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] (ab172974)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7519(2)] to DNA Polymerase lambda/Polk
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)]
See all DNA Polymerase lambda/Polk primary antibodies -
Description
Rabbit monoclonal [EPR7519(2)] to DNA Polymerase lambda/Polk -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
within Human DNA Polymerase lambda/Polk aa 550 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9UGP5 -
Positive control
- WB: A549, HepG2, HeLa and 293T cell lysates.
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General notes
This product was previously labelled as DNA Polymerase lambda.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7519(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] (ab172974)All lanes : Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] (ab172974) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : POLL knockout HeLa cell lysate
Lane 3 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab172974 observed at 75 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab172974 Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] was shown to specifically react with DNA Polymerase lambda/Polk in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264754 (knockout cell lysate ab258595) was used. Wild-type and DNA Polymerase lambda/Polk knockout samples were subjected to SDS-PAGE. ab172974 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] (ab172974)](https://www.abcam.com/ps/products/172/ab172974/Images/ab172974-196091-anti-dna-polymerase-lambda-antibody-epr75192-western-blot.jpg)
Western blot - Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] (ab172974)All lanes : Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] (ab172974) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 63 kDa
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![Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] (ab172974)](https://www.abcam.com/ps/products/172/ab172974/Images/ab172974-3-benefits-of-recombinant-antibodies.png)
Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] (ab172974)
