Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] (ab172974)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7519(2)] to DNA Polymerase lambda/Polk
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)]
See all DNA Polymerase lambda/Polk primary antibodies
Description

Rabbit monoclonal [EPR7519(2)] to DNA Polymerase lambda/Polk
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

within Human DNA Polymerase lambda/Polk aa 550 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9UGP5
Positive control
- WB: A549, HepG2, HeLa and 293T cell lysates.
General notes
This product was previously labelled as DNA Polymerase lambda.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR7519(2)
Isotype

IgG
Research areas

Western blot - Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] (ab172974)

All lanes : Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] (ab172974) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : POLL knockout HeLa cell lysate
Lane 3 : A549 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 63 kDa
Observed band size: 75 kDa
why is the actual band size different from the predicted?

Lanes 1-3: Merged signal (red and green). Green - ab172974 observed at 75 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab172974 Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] was shown to specifically react with DNA Polymerase lambda/Polk in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264754 (knockout cell lysate ab258595) was used. Wild-type and DNA Polymerase lambda/Polk knockout samples were subjected to SDS-PAGE. ab172974 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] (ab172974)

All lanes : Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] (ab172974) at 1/1000 dilution

Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 63 kDa
Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] (ab172974)

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