Recombinant human AMPK alpha1 + AMPK beta1 + AMPK gamma1 protein (Tagged) (ab271362)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, His tag C-Terminus, His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human AMPK alpha1 + AMPK beta1 + AMPK gamma1 protein (Tagged)
See all AMPK alpha1 + AMPK beta1 + AMPK gamma1 proteins and peptides -
Biological activity
Specific Activity: ≥300 pmole/min/µgAssay Conditions: Assay was done in Kinase buffer containing 1 mM DTT using AMARA peptide as a substrate (0.1 mg/ml), 20 μM ATP and 100 μM AMP . Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase- Glo reagent. -
Purity
>= 90 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
MRRLSSWRKMATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTG HKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDI FMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRD LKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRL YAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNP SVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPKYLFPEDPSYSSTMID DEALKEVCEKFECSEEEVLSCLYNRNHQDPLAVAYHLIIDNRRIMNEAKD FYLATSPPDSFLDDHHLTRPHPERVPFLVAETPRARHTLDELNPQKSKHQ GVRKAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWKVVNPYYLRVRRKNP VTSTYSKMSLQLYQVDSRTYLLDFRSIDDEITEAKSGTATPQRSGSVSNY RSCQRSDSDAEAQGKSSEVSLTSSVTSLDSSPVDLTPRPGSHTIEFFEMC ANLIKILAQ -
Molecular weight information
65 kDa by SDS-PAGE ~146 kDa (complex) -
Amino acids
1 to 559 -
Tags
His tag C-Terminus -
Additional sequence information
AMPK alpha1 protein
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Amino Acid Sequence 2
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Species
Human -
Sequence
GNTSSERAALERHGGHKTPRRDSSGGTKDGDRPKILMDSPEDADLFHSEE IKAPEKEEFLAWQHDLEVNDKAPAQARPTVFRWTGGGKEVYLSGSFNNWS KLPLTRSHNNFVAILDLPEGEHQYKFFVDGQWTHDPSEPIVTSQLGTVNN IIQVKKTDFEVFDALMVDSQKCSDVSELSSSPPGPYHQEPYVCKPEERFR APPILPPHLLQVILNKDTGISCDPALLPEPNHVMLNHLYALSIKDGVMVL SATHRYKKKYVTTLLYKPI -
Molecular weight information
31 kDa by SDS-PAGE ~146 kDa (complex) -
Amino acids
2 to 270 -
Tags
His tag C-Terminus
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Amino Acid Sequence 3
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Species
Human -
Sequence
METVISSDSSPAVENEHPQETPESNNSVYTSFMKSHRCYDLIPTSSKLVV FDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRYYK SALVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKI HRLPVIDPESGNTLYILTHKRILKFLKLFITEFPKPEFMSKSLEELQIGT YANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLA AEKTYNNLDVSVTKALQHRSHYFEGVLKCYLHETLETIINRLVEAEVHRL VVVDENDVVKGIVSLSDILQALVLTGGEKKP -
Molecular weight information
38 kDa by SDS-PAGE ~146 kDa (complex) -
Amino acids
1 to 331 -
Tags
His tag C-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 0.82% Sodium phosphate, 1.74% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant Human AMPK alpha1 + AMPK beta1 + AMPK gamma1 protein (Tagged) (ab271362)
Specific activity of ab271362 was ≥300 pmole/min/µg.
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SDS-PAGE - Recombinant Human AMPK alpha1 + AMPK beta1 + AMPK gamma1 protein (Tagged) (ab271362)SDS-PAGE analysis of ab271362.
