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Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106)

Price and availability

274 732 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [16A11C1D4] to HuB+HuC+HuD
  • Suitable for: ELISA, ICC/IF, WB
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-HuB+HuC+HuD antibody [16A11C1D4]
  • Description

    Mouse monoclonal [16A11C1D4] to HuB+HuC+HuD
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human HuB+HuC+HuD aa 233-244 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue).
    Sequence:

    QAQRFRLDNLLN


    Database link: Q12926
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HuC Human recombinant protein fragment His-tagged; HuD Human recombinant protein; SH-SY5Y cell lysate; SH-SY5Y cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% HEPES buffered saline
  • Concentration information loading...
  • Purification notes

    The purity of ab176106 is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation.
  • Clonality

    Monoclonal
  • Clone number

    16A11C1D4
  • Isotype

    IgG2b
  • Light chain type

    kappa

Images

  • Western blot - Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106)
    Western blot - Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106)
    All lanes : Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106) at 1 µg/ml

    Lane 1 : Human HuC protein fragment (ab91911) at 0.1 µg
    Lane 2 : HuD human recombinant protein at 0.1 µg
    Lane 3 : SH-SY5Y cell lysate at 20 µg

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution

    Predicted band size: 40, 42 kDa



    Predicted MW (kDa): HuB/ELAVL2: 39.5 kDa, HuC/ELAVL3: 39.5kDa, HuD/ELAVL4: 41.7kDa

    Why the observed band size is different than the predicted band size: HuC protein (ab91911) is a fragment and has an N terminal His tag and is predicted to present at 24 kDa.

  • Immunocytochemistry/ Immunofluorescence - Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106)
    Immunocytochemistry/ Immunofluorescence - Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106)

    Immunocytochemistry with SH-SY5Y cells stained with ab176106 in red and DAPI in blue, as a counter stain.

    Fixation: Paraformaldehyde fixed (4%, 20 minutes).

    Permeabilization: Triton X-100 permeabilized (0.1%, 10 minutes).

    Blocking: 10% Goat Serum/PBS (1 hour).

    Primary: ab176106, 1 µg/ml (10% Goat Serum/PBS, 2 hours at room temperature or over night at 4°C).

    Secondary: Alexa 594 goat anti-mouse H+L used at a 1/1000 dilution in 1%Goat Serum/PBS for 1hour at RT.

    Washing: 3X 1% Goat Serum/PBS, 10 minutes per wash.

    DAPI: 2 uM in 1% Goat Serum/PBS, 10 minutes.

  • ELISA - Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106)
    ELISA - Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106)

    Specificity of antibody using the ELISA platform.

    Antibody concentration is at 1µg/mL the concentration of recombinant proteins is at 250ng/mL. ab176106 reacts specifically to HuC/ELAVL3 and HuD/ELAVL4 proteins. Reactivity with HuB protein is predicted by the immunogen sequence similarity.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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