Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD44 antibody [EPR1013Y] (ab51037)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1013Y] to CD44
  • Suitable for: IHC-P, WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-CD44 antibody [EPR1013Y]
    See all CD44 primary antibodies

  • Description

    Rabbit monoclonal [EPR1013Y] to CD44

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CD44 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: MDA-MB-231, TF-1, HeLa and A549 cell lysates. Flow Cyt: MDA-MB-231 cells. IHC-P: Pancreatic and cervival cancer, breast and thyroid gland carcinoma, glioma, tonsil and skin tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Flow Cytometry - Anti-CD44 antibody [EPR1013Y] (ab51037)
    Flow Cytometry - Anti-CD44 antibody [EPR1013Y] (ab51037)

    ab51037 used at a 1/200 dilution (red) to detect CD44 in human MDA-MB-231 cells by Flow Cytometry. The secondary used was a Pgoat anti-rabbit IgG (Alexa Fluor® 488) polyclonal used at a 1/2000 dilution. Isotype control (black): Rabbit monoclonal IgG (ab172730). Unlabelled control (Blue): Cell without incubation with primary antibody and secondary antibody.

  • Western blot - Anti-CD44 antibody [EPR1013Y] (ab51037)
    Western blot - Anti-CD44 antibody [EPR1013Y] (ab51037)
    All lanes : Anti-CD44 antibody [EPR1013Y] (ab51037) at 1/5000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : CD44 knockout HeLa cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : LnCAP cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 82 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab51037 observed at 80 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab51037 was shown to react with CD44 in wild-type HeLa cells in western blot. Loss of signal was observed when CD44 knockout sample was used. Wild-type HeLa and CD44 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab51037 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] (ab51037)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] (ab51037)

    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-CD44 antibody [EPR1013Y] (ab51037)
    Western blot - Anti-CD44 antibody [EPR1013Y] (ab51037)
    All lanes : Anti-CD44 antibody [EPR1013Y] (ab51037) at 1/1000 dilution

    Lane 1 : MCF-7 whole cell lysate
    Lane 2 : Jurkat whole cell lysate
    Lane 3 : MDA-MB-231 whole cell lysate
    Lane 4 : HeLa whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 82 kDa
    Observed band size: 81 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and diluting buffer 5% NFDM/TBST

    The expression of CD44 in MCF-7 is low (PMID: 25635866; PMID: 26005723). Jurkat does not express CD44 (PMID: 24127558).

  • Western blot - Anti-CD44 antibody [EPR1013Y] (ab51037)
    Western blot - Anti-CD44 antibody [EPR1013Y] (ab51037)
    Anti-CD44 antibody [EPR1013Y] (ab51037) at 1/5000 dilution + TF-1 cell lysate at 10 µg

    Secondary
    Goat anti-Rabbit HRP labeled. at 1/2000 dilution

    Predicted band size: 82 kDa
    Observed band size: 82 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] (ab51037)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] (ab51037)

    ab51037 (1:100) showing positive staining in human Breast carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] (ab51037)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] (ab51037)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] (ab51037)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] (ab51037)

    Immunohistochemical analysis of paraffin-embedded human skin tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] (ab51037)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] (ab51037)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] (ab51037)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] (ab51037)

    ab51037 (1:100) showing positive staining in human Glioma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] (ab51037)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] (ab51037)

    ab51037 (1:100) showing positive staining in human Thyroid gland carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-CD44 antibody [EPR1013Y] (ab51037)
    OI-RD Scanning - Anti-CD44 antibody [EPR1013Y] (ab51037)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CD44 antibody [EPR1013Y] (ab51037)
    Anti-CD44 antibody [EPR1013Y] (ab51037)

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