Anti-Cleaved Caspase-3 antibody [EPR21032] (ab214430)
![Anti-Cleaved Caspase-3 antibody [EPR21032] (ab214430)](https://www.abcam.com/ps/products/214/ab214430/Images/ab214430-300507-anti-active-caspase-3-antibody-epr21032-western-blot.jpg "Anti-Cleaved Caspase-3 antibody [EPR21032] (ab214430)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21032] to Cleaved Caspase-3
- Suitable for: WB
- Reacts with: Mouse
Overview
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Product name
Anti-Cleaved Caspase-3 antibody [EPR21032]
See all Cleaved Caspase-3 primary antibodies -
Description
Rabbit monoclonal [EPR21032] to Cleaved Caspase-3 -
Host species
Rabbit -
Specificity
ab214430 recognises both pro-Caspase-3 and p17 cleavage fragments. -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: NIH/3T3 cells treated with staurosporine, whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21032 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cleaved Caspase-3 antibody [EPR21032] (ab214430)All lanes : Anti-Cleaved Caspase-3 antibody [EPR21032] (ab214430) at 1/5000 dilution
Lane 1 : Untreated NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : NIH/3T3 cells treated with 1 uM staurosprine for 4 hours, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 31 kDa
Observed band size: 17,19,24,29,32 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
Observed molecular masses are consistent with the literature for full-length and cleaved caspase 3 (PMID: 9922454, PMID 16221205).
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![Anti-Cleaved Caspase-3 antibody [EPR21032] (ab214430)](https://www.abcam.com/ps/products/214/ab214430/Images/ab214430-2-benefits-of-recombinant-antibodies.png)
Anti-Cleaved Caspase-3 antibody [EPR21032] (ab214430)
