Anti-Tuberin (phospho T1462) antibody [EPR1060(2)] (ab109403)
![Anti-Tuberin (phospho T1462) antibody [EPR1060(2)] (ab109403)](https://www.abcam.com/ps/products/109/ab109403/Images/ab109403-122213-anti-tuberin-phospho-t1462-antibody-epr10602-western-blot.jpg "Anti-Tuberin (phospho T1462) antibody [EPR1060(2)] (ab109403)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1060(2)] to Tuberin (phospho T1462)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Tuberin (phospho T1462) antibody [EPR1060(2)]
See all Tuberin primary antibodies -
Description
Rabbit monoclonal [EPR1060(2)] to Tuberin (phospho T1462) -
Host species
Rabbit -
Specificity
ab109403 only detects Tuberin phosphorylated at Threonine 1462. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Phospho specific peptide against residues surrounding Threonine 1462 of Human Tuberin.
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Positive control
- NIH/3T3 cell lysate, Human liver tissue and HeLa cells.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1060(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Tuberin (phospho T1462) antibody [EPR1060(2)] (ab109403)All lanes : Anti-Tuberin (phospho T1462) antibody [EPR1060(2)] (ab109403) at 1/1000 dilution
Lane 1 : NIH/3T3 cell lysate
Lane 2 : NIH/3T3 cell lysate treated with insulin.
Lysates/proteins at 10 µg per lane.
Predicted band size: 201 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuberin (phospho T1462) antibody [EPR1060(2)] (ab109403)](https://www.abcam.com/ps/products/109/ab109403/Images/ab109403-122211-anti-tuberin-phospho-t1462-antibody-epr10602-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuberin (phospho T1462) antibody [EPR1060(2)] (ab109403)Immunohistochemical analysis of paraffin-embedded Human liver tissue using ab109403 at 1/50
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Tuberin (phospho T1462) antibody [EPR1060(2)] (ab109403)](https://www.abcam.com/ps/products/109/ab109403/Images/ab109403-4-benefits-of-recombinant-antibodies.png)
Anti-Tuberin (phospho T1462) antibody [EPR1060(2)] (ab109403)
