Anti-Tuberin antibody (ab245280)
Key features and details
- Rabbit polyclonal to Tuberin
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Tuberin antibody
See all Tuberin primary antibodies -
Description
Rabbit polyclonal to Tuberin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human Tuberin aa 1750-1807. The exact sequence is proprietary.
Database link: P49815 -
Positive control
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245280 was affinity purified using an epitope specific to Tuberin immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Tuberin antibody (ab245280)All lanes : Anti-Tuberin antibody (ab245280) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 201 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-Tuberin antibody (ab245280)Tuberin was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 25% of IP loaded) with ab245280 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245280 at 0.1 µg/ml.
Lane 1: ab245280 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
