Anti-Tuberin (phospho S1254) antibody [EPR5682] (ab133454)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5682] to Tuberin (phospho S1254)
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Tuberin (phospho S1254) antibody [EPR5682]
See all Tuberin primary antibodies -
Description
Rabbit monoclonal [EPR5682] to Tuberin (phospho S1254) -
Host species
Rabbit -
Specificity
ab133454 detects Tuberin only when phosphorylated at Serine 1254. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Phospho specific synthetic peptide corresponding to residues surrounding Serine 1254 of Human Tuberin. (P49815).
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Positive control
- SH-SY5Y cell lysate; Human breast carcinoma tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.03 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5682 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Tuberin (phospho S1254) antibody [EPR5682] (ab133454) at 1/10000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : SH-SY5Y cell lysate treated with Okadaic Acid and Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 201 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
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Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling Tuberin Phospho S1254 with ab133454 at 1/50 dilution
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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