Anti-Tuberin (phospho S939) antibody [EP1062Y] (ab52962)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1062Y] to Tuberin (phospho S939)
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Tuberin (phospho S939) antibody [EP1062Y]
See all Tuberin primary antibodies -
Description
Rabbit monoclonal [EP1062Y] to Tuberin (phospho S939) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman -
Immunogen
Synthetic peptide within Human Tuberin. The exact sequence is proprietary.
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Positive control
- 3T3 cell lysates (untreated or treated with insulin). WB: SH-SY5Y (Human neuroblastoma epithelial cell) treated with 200nM Okadaic Acid (OA) and 1uM Calyculin A (CA) for 60 min whole cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1062Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Tuberin (phospho S939) antibody [EP1062Y] (ab52962) at 1/1000 dilution
Lane 1 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) treated with 200nM Okadaic Acid (OA) and 1uM Calyculin A (CA) for 60 min whole cell lysates
Lane 3 : SH-SY5Y (Human neuroblastoma epithelial cell) treated with 200nM Okadaic Acid (OA) and 1uM Calyculin A (CA) for 60 min whole cell lysates. Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 201 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 90 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
This image is derived from purified batch.
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All lanes : Anti-Tuberin (phospho S939) antibody [EP1062Y] (ab52962) at 1/10000 dilution
Lane 1 : 3T3 cell lysate untreated
Lane 2 : 3T3 cell lysate treated with insulin
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 201 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
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