Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tuberin antibody [EPR22886-235] - BSA and Azide free (ab256472)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22886-235] to Tuberin - BSA and Azide free
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Tuberin antibody [EPR22886-235] - BSA and Azide free
    See all Tuberin primary antibodies

  • Description

    Rabbit monoclonal [EPR22886-235] to Tuberin - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1 (WT), C2C12, RAW 264.7 and HeLa whole cell lysates; human testis tissue lysate; mouse brain tissue lysate. IP: HeLa whole cell lysate.

  • General notes

    Ab256472 is the carrier-free version of ab255612. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256472 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Tuberin antibody [EPR22886-235] - BSA and Azide free (ab256472)
    Western blot - Anti-Tuberin antibody [EPR22886-235] - BSA and Azide free (ab256472)
    All lanes : Anti-Tuberin antibody [EPR22886-235] (ab255612) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : Tuberin knockout HAP1 whole cell lysate
    Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 4 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 5 : A549 (human lung carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 200 kDa
    Observed band size: 200 kDa



    The molecular weight observed is consistent with what has been described in the literature (PMID:30774414; 10335945).

    ab255612 was shown to specifically react with Tuberin in wild-type HAP1 cells as signal was lost in Tuberin knockout cells. Wild-type and Tuberin knockout samples were subjected to SDS-PAGE. ab255612 and ab129002 (Recombinant Anti-Vinculin antibody [EPR8185]) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/20,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposire times.

    Lanes 1-3: 81 seconds; Lane 4: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255612).

  • Immunoprecipitation - Anti-Tuberin antibody [EPR22886-235] - BSA and Azide free (ab256472)
    Immunoprecipitation - Anti-Tuberin antibody [EPR22886-235] - BSA and Azide free (ab256472)

    Tuberin was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg with ab255612 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255612. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg

    Lane 2: ab255612 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab255612 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255612).

  • Anti-Tuberin antibody [EPR22886-235] - BSA and Azide free (ab256472)
    Anti-Tuberin antibody [EPR22886-235] - BSA and Azide free (ab256472)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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