Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034)
Key features and details
- Rabbit polyclonal to 160 kD Neurofilament Medium - Neuronal Marker
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rat, Cow
- Isotype: IgG
Overview
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Product name
Anti-160 kD Neurofilament Medium antibody - Neuronal Marker
See all 160 kD Neurofilament Medium primary antibodies -
Description
Rabbit polyclonal to 160 kD Neurofilament Medium - Neuronal Marker -
Host species
Rabbit -
Specificity
Recognizes the evolutionarily conserved extreme C-terminal region of the medium neurofilament subunit (~145-170 kDa). -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Cow
Predicted to work with: Mouse, Cat, Pig, Bird, Reptile
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Immunogen
Recombinant C-terminal fragment (Rat).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034)ICC/IF image of ab9034 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9034, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034) -
Western blot - Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034)Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034) at 1/20000 dilution + whole rat cerebellum homogenate
A prominent band is observed at ~145kDa corresponding to rat 160kDa Neurofilament. Human and bovine neurofilament medium run a little slower, at about 160kDa. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034)The image illustrates the high definition dendritic staining obtained after protease pretreatment. This picture was kindly supplied as part of the review submitted by Prof Colm Cunningham and Dr Suzanne Campion (Southampton University).
