Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR580(2)Y] to 160 kD Neurofilament Medium (phospho S614 + S619)
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y]
    See all 160 kD Neurofilament Medium primary antibodies

  • Description

    Rabbit monoclonal [EPR580(2)Y] to 160 kD Neurofilament Medium (phospho S614 + S619)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding Serine 614/619 of human 160 kD Neurofilament Medium

  • Positive control

    • SH-SY5Y cell lysates; human brain tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142)

    Immunohistochemical analysis of paraffin-embedded human brain tissue using ab68142 at a dilution of 1/100 - 1/250

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142)
    Western blot - Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142)
    All lanes : Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142) at 1/500 dilution

    Lane 1 : SH-SY5Y cell lysate treated with Alkaline phosphatase
    Lane 2 : Untreated SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated Goat anti-rabbit at 1/2000 dilution

    Predicted band size: 102 kDa
    Observed band size: 160 kDa
    why is the actual band size different from the predicted?

  • Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142)
    Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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