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Anti-Survivin antibody [EPR17358] - BSA and Azide free (ab250577)

Price and availability

526 012 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-Survivin antibody [EPR17358] - BSA and Azide free (ab250577)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17358] to Survivin - BSA and Azide free
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat

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Overview

  • Product name

    Anti-Survivin antibody [EPR17358] - BSA and Azide free
    See all Survivin primary antibodies
  • Description

    Rabbit monoclonal [EPR17358] to Survivin - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250577 is the carrier-free version of ab182132. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250577 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17358
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Survivin / IAPs
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Astrocyte marker
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • IAPs
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • IAPs

Images

  • Western blot - Anti-Survivin antibody [EPR17358] - BSA and Azide free (ab250577)
    Western blot - Anti-Survivin antibody [EPR17358] - BSA and Azide free (ab250577)
    All lanes : Anti-Survivin antibody [EPR17358] (ab182132) at 1/1000 dilution

    Lane 1 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysates
    Lane 2 : Mouse colon lysates
    Lane 4 : C6 (Rat glial tumor cells) whole cell lysates
    Lane 5 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
    Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 16 kDa
    Observed band size: 16 kDa



    This data was developed using ab182132, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR17358] - BSA and Azide free (ab250577)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR17358] - BSA and Azide free (ab250577)

    This data was developed using ab182132, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Survivin with ab182132 at 1/500 dilution followed by Anti-Rabbit HRP (ab97051) secondary at 1/500 dilution. Nuclear staining on mouse spleen is observed. Counter stained with Hematoxylin. Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) secondary at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR17358] - BSA and Azide free (ab250577)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR17358] - BSA and Azide free (ab250577)
    This data was developed using ab182132, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Survivin with ab182132 at 1/500 dilution followed by Anti-Rabbit HRP (ab97051) secondary at 1/500 dilution. Nuclear staining on spermatogenic cells of mouse testis is observed. Counter stained with Hematoxylin. Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) secondary at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-Survivin antibody [EPR17358] - BSA and Azide free (ab250577)
    Immunoprecipitation - Anti-Survivin antibody [EPR17358] - BSA and Azide free (ab250577)
    This data was developed using ab182132, the same antibody clone in a different buffer formulation.Survivin was immunoprecipitated from 1mg of F9 (Mouse embyro testicular cancer cell line) whole cell extract with ab182132 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab182132 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: F9 whole cell extract. Lane 2: PBS instead of F9 whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  • Anti-Survivin antibody [EPR17358] - BSA and Azide free (ab250577)
    Anti-Survivin antibody [EPR17358] - BSA and Azide free (ab250577)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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