Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4644(2)] to IRF2 - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free
    See all IRF2 primary antibodies

  • Description

    Rabbit monoclonal [EPR4644(2)] to IRF2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SW480, HeLa, Jurkat, Caco-2, RAW264.7 and NIH/3T3 cell lysates, and Human fetal lung lysate. IHC-P: Human colon, Human cervical cancer, Mouse colon and human colonic carcinoma tissue. ICC/IF: HeLa cells.

  • General notes

    ab229443 is the carrier-free version of ab124744 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab229443 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)
    Western blot - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)

    This WB data was generated using the same anti-IRF2 antibody clone, EPR4644(2), in a different buffer formulation (cat# ab12474).

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: IRF2 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: CACO2 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab124744 observed at 48 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab124744 was shown to recognize IRF2 when IRF2 knockout samples were used, along with additional cross-reactive bands. Wild-type and IRF2 knockout samples were subjected to SDS-PAGE.  Ab124744 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 and 1/10000 dilutions respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)
    Immunocytochemistry/ Immunofluorescence - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling IRF2 with ab124744 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cells.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab124744 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124744).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue labeling IRF2 with purified ab124744 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution was used as the secondary antibody. Nucleus staining on epithelial cells of mouse colon was observed. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124744).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervical cancer tissue labeling IRF2 with purified ab124744 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution was used as the secondary antibody. Nucleus staining on tumor cells of human cervix cancer was observed. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124744).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colonic carcinoma tissue labelling IRF2 with purified ab124744 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124744).

  • OI-RD Scanning - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)
    OI-RD Scanning - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124744).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)

    This IHC data was generated using the same anti-IRF2 antibody clone, EPR4644(2), in a different buffer formulation (cat# ab124744).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue labeling IRF2 with purified ab124744 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution was used as the secondary antibody. Nucleus staining on epithelium of human colon was observed. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.

  • Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)
    Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)

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