Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)
Key features and details
- Rabbit polyclonal to alpha Tubulin - Microtubule Marker
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-alpha Tubulin antibody - Microtubule Marker
See all alpha Tubulin primary antibodies -
Description
Rabbit polyclonal to alpha Tubulin - Microtubule Marker -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human alpha Tubulin aa 400 to the C-terminus. The exact sequence is proprietary.
Database link: Q6PEY2 -
General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)
Lanes: 1, 4, 7 - SK N BE; 2, 5, 8 - TE 671; 3, 6, 9 - HeLa
Lanes: 1-3: 4oC (1 freeze/thaw); 4-6: 4oC (2 freeze/thaws); 7-9 : 4oC (4 freeze/thaws)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)ab15246 at 1/200 dilution, staining alpha Tubulin in Human lung tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin- embedded tissue sections).
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Western blot - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)All lanes : Anti-alpha Tubulin antibody - Microtubule Marker (ab15246) at 1 µg/ml
Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 50 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
