Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Tuesday March 02, 2021

Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13478(B)] to alpha Tubulin - Loading Control
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control
See all alpha Tubulin primary antibodies
Description

Rabbit monoclonal [EPR13478(B)] to alpha Tubulin - Loading Control
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human alpha Tubulin aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P68366
Positive control
- HeLa, Jurkat, A431 and K562 cell lysates; Human kidney and uterus tissues; A431 and Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13478(B)
Isotype

IgG
Research areas

Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560) at 1/7000 dilution (Purified) + Jurkat cell lysate at 10 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue labeling alpha Tubulin with unpurified ab176560 at a 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

Immunohistochemical staining of paraffin embedded human kidney with purified ab176560 at a dilution of 1/350. A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was counter stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

Immunofluorescent staining of A431 cells fixed in 4% PFA with purified ab176560 at a dilution of 1/350. An Alexa Fluor^® 555 goat anti-rabbit was used as the secondary at a dilution of 1/500 and the sample was counter stained with DAPI. An Alexa Fluor^® 555 goat anti-mouse was used at a dilution of 1/500 as the negative control and is shown in the bottom right hand panel.
Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

All lanes : Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560) at 1/1000 dilution (Unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : A431 cell lysate
Lane 4 : K562 cell lysate

Lysates/proteins at 10 µg per lane.

Developed using the ECL technique.

Predicted band size: 50 kDa

Secondary antibodies - anti-rabbit HRP (ab6721)
Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

Immunofluorescent staining of A431 cells fixed in 4% PFA with unpurified ab176560 at a dilution of 1/100. An Alexa Fluor^® 555 goat anti-rabbit was used as the secondary at a dilution of 1/500 and the sample was counter stained with DAPI. An Alexa Fluor^® 555 goat anti-mouse was used at a dilution of 1/500 as the negative control and is shown in the bottom right hand panel.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

Immunohistochemical staining of paraffin embedded human kidney with unpurified ab176560 at a dilution of 1/100. A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was counter stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cytometry - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

Flow cytometry analysis of permeabilized HeLa cells labeling alpha Tubulin (pink) with purified ab176560 at a 1/70 dilution, or negative control rabbit IgG (green). The secondary antibody was FITC goat anti-rabbit.
Flow Cytometry - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

Flow cytometry analysis of permeabilized Jurkat cells labeling alpha Tubulin (red) with unpurified ab176560 at a 1/10 dilution, or negative control rabbit IgG (green)
Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

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