Anti-Doublecortin antibody [2G5] (ab135349)
Key features and details
- Mouse monoclonal [2G5] to Doublecortin
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Doublecortin antibody [2G5]
See all Doublecortin primary antibodies -
Description
Mouse monoclonal [2G5] to Doublecortin -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB MouseRecombinant fragment -
Immunogen
Recombinant fragment, corresponding to amino acids 362-411 of Human Doublecortin expressed in E. Coli.
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Positive control
- Human Doublecortin recombinant protein; Mouse heart lysate; HepG2 and SK-N-SH cells; Human brain and kidney cancer tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Contains 0.5% protein stabilizer. -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2G5 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling Doublecortin with ab135349 at 1/200 dilution followed by DAB staining.
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Flow cytometric analysis of SK-N-SH cells labelling Doublecortin with ab135349 at 1/200 dilution (green) or negative control (red).
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Immunofluorescent analysis of HepG2 cells labelling Doublecortin with ab135349 at 1/200 dilution (green). DRAQ5 fluorescent DNA dye (blue).
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Immunohistochemical analysis of paraffin-embedded Human kidney cancer tissue labelling Doublecortin with ab135349 at 1/200 dilution followed by DAB staining.
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Anti-Doublecortin antibody [2G5] (ab135349) at 1/500 dilution + Mouse heart lysate
Predicted band size: 49 kDa
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Anti-Doublecortin antibody [2G5] (ab135349) at 1/500 dilution + Recombinant Human Doublecortin protein
Predicted band size: 49 kDa
Expected MW of recombinant protein is 34.1 kDa.