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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules

Anti-Doublecortin antibody [2G5] (ab135349)

Price and availability

274 732 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-Doublecortin antibody [2G5] (ab135349)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [2G5] to Doublecortin
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Doublecortin antibody [2G5]
    See all Doublecortin primary antibodies
  • Description

    Mouse monoclonal [2G5] to Doublecortin
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant fragment, corresponding to amino acids 362-411 of Human Doublecortin expressed in E. Coli.

  • Positive control

    • Human Doublecortin recombinant protein; Mouse heart lysate; HepG2 and SK-N-SH cells; Human brain and kidney cancer tissues.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS

    Contains 0.5% protein stabilizer.
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    2G5
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Microtubules
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Growth Cone
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Neuronal

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [2G5] (ab135349)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [2G5] (ab135349)
    Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling Doublecortin with ab135349 at 1/200 dilution followed by DAB staining.
  • Flow Cytometry - Anti-Doublecortin antibody [2G5] (ab135349)
    Flow Cytometry - Anti-Doublecortin antibody [2G5] (ab135349)
    Flow cytometric analysis of SK-N-SH cells labelling Doublecortin with ab135349 at 1/200 dilution (green) or negative control (red).
  • Immunocytochemistry/ Immunofluorescence - Anti-Doublecortin antibody [2G5] (ab135349)
    Immunocytochemistry/ Immunofluorescence - Anti-Doublecortin antibody [2G5] (ab135349)
    Immunofluorescent analysis of HepG2 cells labelling Doublecortin with ab135349 at 1/200 dilution (green). DRAQ5 fluorescent DNA dye (blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [2G5] (ab135349)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [2G5] (ab135349)
    Immunohistochemical analysis of paraffin-embedded Human kidney cancer tissue labelling Doublecortin with ab135349 at 1/200 dilution followed by DAB staining.
  • Western blot - Anti-Doublecortin antibody [2G5] (ab135349)
    Western blot - Anti-Doublecortin antibody [2G5] (ab135349)
    Anti-Doublecortin antibody [2G5] (ab135349) at 1/500 dilution + Mouse heart lysate

    Predicted band size: 49 kDa

  • Western blot - Anti-Doublecortin antibody [2G5] (ab135349)
    Western blot - Anti-Doublecortin antibody [2G5] (ab135349)
    Anti-Doublecortin antibody [2G5] (ab135349) at 1/500 dilution + Recombinant Human Doublecortin protein

    Predicted band size: 49 kDa



    Expected MW of recombinant protein is 34.1 kDa.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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