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PE Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab208752)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Overview

  • Product name

    PE Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker
    See all alpha Tubulin primary antibodies

  • Description

    PE Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker

  • Host species

    Rabbit

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: HeLa cells. ICC/IF: MCF-7 cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - PE Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab208752)
    Flow Cytometry - PE Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab208752)

    Overlay histogram showing HeLa cells stained with ab208752 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol (-20°C) for 30 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab208750, 1/2500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycorythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20 mW Solid State Blue Laser (488nm) and 585/40 bandpass filter.

  • Immunocytochemistry/ Immunofluorescence - PE Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab208752)
    Immunocytochemistry/ Immunofluorescence - PE Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab208752)

    ab208752 staining alpha Tubulin in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208752 at 1/500 dilution (Pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • PE Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab208752)
    PE Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab208752)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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