Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1568Y] to beta III Tubulin - BSA and Azide free
  • Suitable for: IHC-P, ICC, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free
    See all beta III Tubulin primary antibodies

  • Description

    Rabbit monoclonal [EPR1568Y] to beta III Tubulin - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    According to BLAST analysis, it is possible that the antibody will cross-react with TBB6 (Q9BUF5), TBB2B (Q9BVA1), TBB2A (Q13885), TBB5 (P07437), TBB4A (P04350), TBB4B (P68371). This cross-reactivity has not been confirmed experimentally.

  • Tested applications

    Suitable for: IHC-P, ICC, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247375 is the carrier-free version of ab68193. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247375 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR1568Y

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)
    Immunocytochemistry - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)

    This data was developed using ab68193, the same antibody clone in a different buffer formulation.

    ab68193 staining beta III Tubulin in SKNSH cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab18207 at 5μg/ml and ab7291 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150081) at 2 μg/ml (shown in green) and AlexaFluor®594 Goat anti-Mouse secondary (ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.
    Negative controls: 1– Rabbit primary and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.
  • Immunocytochemistry - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)
    Immunocytochemistry - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)
    This data was developed using ab68193, the same antibody clone in a different buffer formulation.ab68193 staining beta III Tubulin in Neuro-2a cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab68193 at 1μg/ml and ab7291 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150081) at 2 μg/ml (shown in green) and AlexaFluor®594 Goat anti-Mouse secondary (ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.
    Negative controls: 1– Rabbit primary and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.
  • Western blot - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)
    Western blot - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)
    All lanes : Anti-beta III Tubulin antibody [EPR1568Y] (ab68193) at 1/20000 dilution

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Spinal Cord (Mouse) Tissue Lysate
    Lane 4 : Spinal Cord (Rat) Tissue Lysate
    Lane 5 : PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

    Predicted band size: 50 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab68193, the same antibody clone in a different buffer formulation.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab68193 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  • Flow Cytometry - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)
    Flow Cytometry - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)
    This data was developed using ab68193, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab68193 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab68193, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was anti-rabbit Alexa Fluor® 488 (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions.
  • Western blot - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)
    Western blot - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)
    Anti-beta III Tubulin antibody [EPR1568Y] (ab68193) at 1/50000 dilution + HeLa cell lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    This data was developed using ab68193, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)
    This data was developed using ab68193, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human brain tissue using ab68193 at 1/250-1/500 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)
    Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)

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