Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Detyrosinated alpha Tubulin antibody [AA12] - BSA and Azide free (ab255906)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [AA12] to Detyrosinated alpha Tubulin - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Detyrosinated alpha Tubulin antibody [AA12] - BSA and Azide free
    See all Detyrosinated alpha Tubulin primary antibodies

  • Description

    Mouse monoclonal [AA12] to Detyrosinated alpha Tubulin - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human, rat and mouse brain tissue lysate. Human and rat hippocampus tissue lysate. IHC-P: Human, mouse and rat cerebrum tissue. IHC-Fr: Mouse and rat cerebrum tissue. ICC/IF: Mouse primary neuron cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255906 is the carrier-free version of ab254154. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    AA12

  • Isotype

    IgG2a

  • Research areas

Images

  • Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] - BSA and Azide free (ab255906)
    Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] - BSA and Azide free (ab255906)
    All lanes : Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

    Predicted band size: 50 kDa


    Exposure time: 7 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Negative control: Mouse heart (PMID:15899979).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254154).

  • Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] - BSA and Azide free (ab255906)
    Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] - BSA and Azide free (ab255906)
    All lanes : Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154) at 1/1000 dilution

    Lane 1 : Human hippocampus tissue lysate
    Lane 2 : Human brain tissue lysate
    Lane 3 : Rat hippocampus tissue lysate
    Lane 4 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
    Lanes 3-4 : Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

    Predicted band size: 50 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1, 2: 3 seconds; Lane 3, 4: 7 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254154).

  • Immunohistochemistry (Frozen sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - BSA and Azide free (ab255906)
    Immunohistochemistry (Frozen sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - BSA and Azide free (ab255906)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/100 dilution followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 (2µg/ml) dilution (Green). Cytoplasmic staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 (2µg/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254154).

  • Immunohistochemistry (Frozen sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - BSA and Azide free (ab255906)
    Immunohistochemistry (Frozen sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - BSA and Azide free (ab255906)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/100 dilution followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 (2µg/ml) dilution (Green). Cytoplasmic staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488)at 1/1000 (2µg/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254154).

  • Immunocytochemistry/ Immunofluorescence - Anti-Detyrosinated alpha Tubulin antibody [AA12] - BSA and Azide free (ab255906)
    Immunocytochemistry/ Immunofluorescence - Anti-Detyrosinated alpha Tubulin antibody [AA12] - BSA and Azide free (ab255906)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neuron cells labelling Detyrosinated A Tubulin with ab254154 at 1/250 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron cells. Confocal scanning Z step was set as 0.3µm followed by image processing with maximum Z projection. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (Red). The nuclear counterstain was DAPI (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254154).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - BSA and Azide free (ab255906)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - BSA and Azide free (ab255906)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on rat cerebrum. The section was incubated with ab254154 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254154).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - BSA and Azide free (ab255906)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - BSA and Azide free (ab255906)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on mouse cerebrum. The section was incubated with ab254154 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254154).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - BSA and Azide free (ab255906)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - BSA and Azide free (ab255906)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human cerebrum. The section was incubated with ab254154 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254154).

  • Anti-Detyrosinated alpha Tubulin antibody [AA12] - BSA and Azide free (ab255906)
    Anti-Detyrosinated alpha Tubulin antibody [AA12] - BSA and Azide free (ab255906)

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