Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TSC22 domain family, member 4 antibody [EPR14789] - C-terminal (ab190349)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14789] to TSC22 domain family, member 4 - C-terminal
  • Suitable for: IP, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TSC22 domain family, member 4 antibody [EPR14789] - C-terminal
    See all TSC22 domain family, member 4 primary antibodies

  • Description

    Rabbit monoclonal [EPR14789] to TSC22 domain family, member 4 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, SK-OV-3, K562, mouse RAW 264.7, mouse NIH 3T3 and rat PC12 cell lysates. HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14789

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-TSC22 domain family, member 4 antibody [EPR14789] - C-terminal (ab190349)
    Western blot - Anti-TSC22 domain family, member 4 antibody [EPR14789] - C-terminal (ab190349)
    All lanes : Anti-TSC22 domain family, member 4 antibody [EPR14789] - C-terminal (ab190349) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : SK-OV-3 cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

  • Western blot - Anti-TSC22 domain family, member 4 antibody [EPR14789] - C-terminal (ab190349)
    Western blot - Anti-TSC22 domain family, member 4 antibody [EPR14789] - C-terminal (ab190349)
    All lanes : Anti-TSC22 domain family, member 4 antibody [EPR14789] - C-terminal (ab190349) at 1/1000 dilution

    Lane 1 : mouse RAW 264.7 cell lysate
    Lane 2 : rat PC12 cell lysate
    Lane 3 : mouse NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

  • Immunoprecipitation - Anti-TSC22 domain family, member 4 antibody [EPR14789] - C-terminal (ab190349)
    Immunoprecipitation - Anti-TSC22 domain family, member 4 antibody [EPR14789] - C-terminal (ab190349)

    Western blot analysis on immunoprecipitation from 1) HeLa cell lysate and 2) PBS, labeling TSC22 domain family, member 4 using ab190349 at 1/50 dilution and HRP-conjugated anti-rabbit IgG specific to the non-reduced form of rabbit IgG at a 1/1500 dilution.

  • Flow Cytometry - Anti-TSC22 domain family, member 4 antibody [EPR14789] - C-terminal (ab190349)
    Flow Cytometry - Anti-TSC22 domain family, member 4 antibody [EPR14789] - C-terminal (ab190349)

    Flow cytometry analysis of paraformaldehyde-fixed HeLa cells labeling TSC22 domain family, member 4 with ab190349 at a 1/50 dilution (pink) or a rabbit IgG isotype control (green), followed Goat anti rabbit IgG (FITC) at a 1/150 dilution.

  • Anti-TSC22 domain family, member 4 antibody [EPR14789] - C-terminal (ab190349)
    Anti-TSC22 domain family, member 4 antibody [EPR14789] - C-terminal (ab190349)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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