Anti-TSC22 domain family, member 4 antibody [EPR14789] - C-terminal (ab190349)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14789] to TSC22 domain family, member 4 - C-terminal
- Suitable for: IP, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-TSC22 domain family, member 4 antibody [EPR14789] - C-terminal
See all TSC22 domain family, member 4 primary antibodies -
Description
Rabbit monoclonal [EPR14789] to TSC22 domain family, member 4 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIP HumanWB MouseHuman -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, SK-OV-3, K562, mouse RAW 264.7, mouse NIH 3T3 and rat PC12 cell lysates. HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14789 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-TSC22 domain family, member 4 antibody [EPR14789] - C-terminal (ab190349) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human fetal liver lysate
Lane 3 : SK-OV-3 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 41 kDa
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All lanes : Anti-TSC22 domain family, member 4 antibody [EPR14789] - C-terminal (ab190349) at 1/1000 dilution
Lane 1 : mouse RAW 264.7 cell lysate
Lane 2 : rat PC12 cell lysate
Lane 3 : mouse NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 41 kDa
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Western blot analysis on immunoprecipitation from 1) HeLa cell lysate and 2) PBS, labeling TSC22 domain family, member 4 using ab190349 at 1/50 dilution and HRP-conjugated anti-rabbit IgG specific to the non-reduced form of rabbit IgG at a 1/1500 dilution.
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Flow cytometry analysis of paraformaldehyde-fixed HeLa cells labeling TSC22 domain family, member 4 with ab190349 at a 1/50 dilution (pink) or a rabbit IgG isotype control (green), followed Goat anti rabbit IgG (FITC) at a 1/150 dilution.
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