Anti-beta Tubulin antibody [AA2] (ab231082)
![Anti-beta Tubulin antibody [AA2] (ab231082)](https://www.abcam.com/ps/products/231/ab231082/Images/ab231082-320369-anti-beta-tubulin-antibody-aa2-western-blot.jpg "Anti-beta Tubulin antibody [AA2] (ab231082)")
Key features and details
- Mouse monoclonal [AA2] to beta Tubulin
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-beta Tubulin antibody [AA2]
See all beta Tubulin primary antibodies -
Description
Mouse monoclonal [AA2] to beta Tubulin -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB MouseRatHuman
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Immunogen
Full length protein corresponding to Cow beta Tubulin. (Bovine brain tubulin).
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Epitope
Recognizes amino acids 416-431 -
Positive control
- ICC/IF: A431 cells. IHC-P: FFPE human colon carcinoma tissue sections. WB: HeLa, NIH3T3, PC12
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General notes
This antibody clone is manufactured by Abcam. If you require it in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
AA2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-beta Tubulin antibody [AA2] (ab231082)All lanes :
Lane 1 : HeLa whole cell lysate
Lane 2 : NIH3T3 whole cell lysate
Lane 3 : PC12 whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under non-reducing conditions.
Predicted band size: 49 kDaThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab231082 and ab9485 (Rabbit anti-GAPDH loading control) overnight at 4°C at 1ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [AA2] (ab231082)](https://www.abcam.com/ps/products/231/ab231082/Images/ab231082-319898-anti-beta-tubulin-antibody-aa2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [AA2] (ab231082)ab231082 staining beta Tubulin in A431 (human epidermoid carcinoma cell line) cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab231082 at 1μg/ml. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [AA2] (ab231082)](https://www.abcam.com/ps/products/231/ab231082/Images/ab231082-320135-anti-beta-tubulin-antibody-aa2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [AA2] (ab231082)IHC image of beta tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon carcinoma* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab231082, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
