Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1331Y] to beta Tubulin - Microtubule Marker
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker
    See all beta Tubulin primary antibodies

  • Description

    Rabbit monoclonal [EP1331Y] to beta Tubulin - Microtubule Marker

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human beta Tubulin aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P07437

  • Positive control

    • WB: PC12 and HeLa whole cell lysate (ab150035) and mouse brain, rat brain, mouse spinal cord and rat spinal cord tissue lysates. IHC-P: Human gastric carcinoma. ICC/IF: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)
    Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)
    All lanes : Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901) at 1/20000 dilution

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Spinal Cord (Mouse) Tissue Lysate
    Lane 4 : Spinal Cord (Rat) Tissue Lysate
    Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

    Predicted band size: 50 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?



    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab52901 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  • Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)
    Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)
    Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901) at 1/20000 dilution + HeLa cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)

    ab52901 at 1/250 dilution staining beta Tubulin in human gastric carcinoma by Immunohistochemsitry, Paraffin embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)
    Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901) This image is courtesy of an anonymous Abreview
    Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901) at 1/1000 dilution (in PBS +0.5% Tween20 for 2 hours at 23°C) + 293 human embryonic kidney whole cell lysate at 25 µg

    Secondary
    An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?


    Exposure time: 45 seconds


    Blocking Step: 5% Milk for 1 hour at 23°C

    See Abreview

  • Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)
    Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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