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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules

Anti-Tubulin antibody [EPR13796] - BSA and Azide free (ab251523)

Anti-Tubulin antibody [EPR13796] - BSA and Azide free (ab251523)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13796] to Tubulin - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Saccharomyces cerevisiae

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Overview

  • Product name

    Anti-Tubulin antibody [EPR13796] - BSA and Azide free
    See all Tubulin primary antibodies
  • Description

    Rabbit monoclonal [EPR13796] to Tubulin - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody recognizes Tubulin alpha-1 and alpha-3 chains.

  • Tested Applications & Species

    Application Species
    WB
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251523 is the carrier-free version of ab210797. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251523 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR13796
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker

Images

  • Western blot - Anti-Tubulin antibody [EPR13796] - BSA and Azide free (ab251523)
    Western blot - Anti-Tubulin antibody [EPR13796] - BSA and Azide free (ab251523)
    Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797) at 1/1000 dilution + Saccharomyces cerevisiae whole cell lysates at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa


    Exposure time: 3 minutes


    This data was developed using ab210797, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Tubulin antibody [EPR13796] - BSA and Azide free (ab251523)
    Western blot - Anti-Tubulin antibody [EPR13796] - BSA and Azide free (ab251523)
    Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797) at 1/1000 dilution + Full length recombinant protein Saccharomyces cerevisiae tubulin alpha-3 chain at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa


    Exposure time: 3 minutes


    This data was developed using ab210797, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Full length recombinant protein Saccharomyces cerevisiae tubulin alpha-3 chain contains aa1-445 with a His-Tag® was made in house.

  • Anti-Tubulin antibody [EPR13796] - BSA and Azide free (ab251523)
    Anti-Tubulin antibody [EPR13796] - BSA and Azide free (ab251523)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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