Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/1000 dilution + Human beta I Tubulin recombinant protein at 0.01 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

 

The immunogen of ab179513 has 67% identities with beta I Tubulin. The WB image shows it cross-reacts with beta I Tubulin.
Human beta I Tubulin is an in house recombinant protein (aa1-451) with a proprietary tag.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling beta Tubulin with ab179513 at 1/1000 dilution, followed by anti-rabbit Alexa Fluor® 488 (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and anti-mouse AlexaFluor® 594 (ab150120) at 1/500 dilution (red).
The negative controls are as follows;
-ve control 1: ab179513 at 1/1000 dilution followed by anti-mouse AlexaFluor® 594 (ab150120) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by anti-rabbit Alexa Fluor® 488 (ab150077) at 1/500 dilution.

 

Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling beta Tubulin with ab179513 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

All lanes : Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/2000 dilution

Lane 1 : Zebrafish whole lysates
Lane 2 : Xenopus tropicalis lysates
Lane 3 : Drosophila whole lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

 

The amino acid sequence of ab179513 immunogen is identical to those of tubulin beta 2A, 2B, 3, 4A, 4B, 5, 6 and 8 at the same region.

All lanes : Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/2000 dilution

Lane 1 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates
Lane 2 : MDCK (Canine kidney cell line) whole cell lysates
Lane 3 : MDBK (Bovine kidney cell line) whole cell lysates
Lane 4 : COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

 

The amino acid sequence of ab179513 immunogen is identical to those of tubulin beta 2A, 2B, 3, 4A, 4B, 5, 6 and 8 at the same region.

All lanes : Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/2000 dilution

Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
Lane 3 : A431 (Human epidermoid carcinoma) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

 

The amino acid sequence of ab179513 immunogen is identical to those of tubulin beta 2A, 2B, 3, 4A, 4B, 5, 6 and 8 at the same region.

All lanes : Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/2000 dilution

Lane 1 : Human fetal brain lysates
Lane 2 : Human fetal kidney lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

 

The amino acid sequence of ab179513 immunogen is identical to those of tubulin beta 2A, 2B, 3, 4A, 4B, 5, 6 and 8 at the same region.

All lanes : Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/2000 dilution

Lane 1 : Mouse brain lysates
Lane 2 : Rat brain lysates
Lane 3 : C6 (Rat glial tumor cells) whole cell lysates
Lane 4 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 6 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

 

The amino acid sequence of ab179513 immunogen is identical to those of tubulin beta 2A, 2B, 3, 4A, 4B, 5, 6 and 8 at the same region.

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining on neurons of human cerebral cortex is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining on tubules and the glomerulus of human kidney is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining on human glioma is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasm staining on neurons of mouse cerebral cortex is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasm staining on neurons of rat cerebral cortex is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.