Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SOX10 antibody [SP267] - BSA and Azide free (ab245760)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP267] to SOX10 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, IHC-P, WB, IHC-FoFr
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-SOX10 antibody [SP267] - BSA and Azide free
    See all SOX10 primary antibodies

  • Description

    Rabbit monoclonal [SP267] to SOX10 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IHC-FoFr
    Mouse
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A-375 cell lysate. IHC-P: Human melanoma tissue. Flow Cyt: A375, C6, and B16-F0 cells. ICC/IF: A375, C6, and B16-F0 cells. IHC-Fr: Mouse cerebellum

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Ab245760 is the carrier-free version of ab227680. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab245760 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP267] - BSA and Azide free (ab245760)
    Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP267] - BSA and Azide free (ab245760)
    Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling SOX10 with purified ab227680 at 1:25 (3 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227680).
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-SOX10 antibody [SP267] - BSA and Azide free (ab245760)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-SOX10 antibody [SP267] - BSA and Azide free (ab245760)

    Immunohistochemistry (Frozen) analysis of mouse cerebellum tissue sections labeling SOX10 with purified ab227680 at 1/50 (1.4 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. Sections were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Negative control: PBS instead of the primary antibody. DAPI (blue) was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide ab227680).

  • Flow Cytometry - Anti-SOX10 antibody [SP267] - BSA and Azide free (ab245760)
    Flow Cytometry - Anti-SOX10 antibody [SP267] - BSA and Azide free (ab245760)

    Flow Cytometry analysis of C6 (Rat glial tumor glial cell) cells labeling SOX10 with purified ab227680 at 1:20 dilution (3.75 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227680).

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP267] - BSA and Azide free (ab245760)
    Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP267] - BSA and Azide free (ab245760)
    Immunocytochemistry/ Immunofluorescence analysis of B16-F0 ( mouse melanoma epithelial cell-like) cells labeling SOX10 with purified ab227680 at 1:25 (3 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab130748).
  • Flow Cytometry - Anti-SOX10 antibody [SP267] - BSA and Azide free (ab245760)
    Flow Cytometry - Anti-SOX10 antibody [SP267] - BSA and Azide free (ab245760)

    Flow Cytometry analysis of B16-F0 (Mouse melanoma epithelial cell-like) cells labeling SOX10 with purified ab227680 at 1:200 dilution (0.375 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227646).

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP267] - BSA and Azide free (ab245760)
    Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP267] - BSA and Azide free (ab245760)

    Immunocytochemistry/ Immunofluorescence analysis of A-375 (human malignant melanoma epithelial cell) cells labeling SOX10 with purified ab227680 at 1:25 (3 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227680).

  • Flow Cytometry - Anti-SOX10 antibody [SP267] - BSA and Azide free (ab245760)
    Flow Cytometry - Anti-SOX10 antibody [SP267] - BSA and Azide free (ab245760)

    Flow Cytometry analysis of A-375 (Human malignant melanoma epithelial cell) cells labeling SOX10 with purified ab227680 at 1:200 dilution (0.375 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227680).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP267] - BSA and Azide free (ab245760)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP267] - BSA and Azide free (ab245760)

    Formalin-fixed, paraffin-embedded human melanoma tissue stained for SOX10 using ab227680 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227680).

  • Anti-SOX10 antibody [SP267] - BSA and Azide free (ab245760)
    Anti-SOX10 antibody [SP267] - BSA and Azide free (ab245760)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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