Anti-SOX10 antibody (ab27655)
Key features and details
- Rabbit polyclonal to SOX10
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Chicken
- Isotype: IgG
Overview
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Product name
Anti-SOX10 antibody
See all SOX10 primary antibodies -
Description
Rabbit polyclonal to SOX10 -
Host species
Rabbit -
Specificity
Antibody was raised using a synthetic peptide corresponding to Sox8. However, this antibody demonstrates cross reactivity against Sox10. Does not cross react with SOX9 -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P ChickenWB Mouse
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Immunogen
Synthetic peptide:
CPPAHSPTANWDQPVYTTLT
, corresponding to C terminal amino acids 449-468 of Chicken SOX8
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody (ab27655)Sections through the trunk of E4 chick embryos fixed in 4 % paraformaldehyde and stained with anti-SOX10 (ab27655) diluted 1/5000. Secondary antibody was FITC-conjugated donkey anti-rabbit. ab27655 stains the embryonic neural crest cells which can be seen migrating into the dorsal root ganglion.
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Western blot - Anti-SOX10 antibody (ab27655)All lanes : Anti-SOX10 antibody (ab27655) at 1/500 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 74 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
SOX10 contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
