Anti-SNAIL + SLUG antibody (ab85936)
Key features and details
- Rabbit polyclonal to SNAIL + SLUG
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-SNAIL + SLUG antibody
See all SNAIL + SLUG primary antibodies -
Description
Rabbit polyclonal to SNAIL + SLUG -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: Rat
Does not react with:
Mouse -
Immunogen
Synthetic peptide corresponding to Human SNAIL + SLUG aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab19126) -
Positive control
- WB: Human SNAIL Full-length Recombinant Protein (Tagged). IHC-P: Human breast ductal carcinoma.
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General notes
Batches sold under ab85936 have passed QC testing in Western Blot (Recombinant only) and IHC. In our hands, this product did not work in ICC/IF. For further information please contact our Customer Services team.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab85936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. Please note this antibody has only been shown to detect recombinant protein and we are unable to detect endogenous protein by western blot, in our lab. Please contact our scientific support services if you have any queries regarding this antibody. IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Target
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Relevance
Function: SNAIL is involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm (By similarity). Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. SLUG is a transcriptional repressor, involved in the generation and migration of neural crest cells. PTM: SNAIL is phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Similarity: Both SNAIL and SLUG belong to the snail C2H2-type zinc-finger protein family. Tissue specificity: SNAIL is expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. SLUG is expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle. -
Cellular localization
Slug is generally nuclear, while Snail is known to be both cytoplasmic and nuclear. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) snail is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs. -
Database links
- Entrez Gene: 6591 Human
- Entrez Gene: 6615 Human
- Entrez Gene: 116490 Rat
- Entrez Gene: 25554 Rat
- Omim: 604238 Human
- SwissProt: O43623 Human
- SwissProt: O95863 Human
- SwissProt: O08954 Rat
see all -
Alternative names
- dJ710H13.1 antibody
- MGC10182 antibody
- Neural crest transcription factor Slug antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody (ab85936)IHC image of ab85936 staining in human breast ductal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85936, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-SNAIL + SLUG antibody (ab85936)All lanes : Anti-SNAIL + SLUG antibody (ab85936) at 1 µg/ml
Lane 1 : SLUG (SNAI2) Human - GST Tagged Recombinant Protein
Lane 2 : SNAIL (SNAI1) - GST Tagged Recombinant Protein
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Please note this antibody has only been shown to detect recombinant protein and we are unable to detect endogenous protein by western blot, in our lab. Please contact our scientific support services if you have any queries regarding this antibody.
Protocols
References (21)
ab85936 has been referenced in 21 publications.
- Sun R et al. Expression of BMP7 in cervical cancer and inhibition of epithelial-mesenchymal transition by BMP7 knockdown in HeLa cells. Int J Mol Med 45:1417-1424 (2020). PubMed: 32323730
- Xu L et al. ATM deficiency promotes progression of CRPC by enhancing Warburg effect. Endocr Relat Cancer 26:59-71 (2019). PubMed: 30400006
- Osio A et al. EGFR is involved in dermatofibrosarcoma protuberans progression to high grade sarcoma. Oncotarget 9:8478-8488 (2018). PubMed: 29492209
- Leo C et al. Overexpression of Lox in triple-negative breast cancer. Ann Diagn Pathol 34:98-102 (2018). IHC-P ; Human . PubMed: 29661738
- Peng YP et al. PIK3R3 Promotes Metastasis of Pancreatic Cancer via ZEB1 Induced Epithelial-Mesenchymal Transition. Cell Physiol Biochem 46:1930-1938 (2018). PubMed: 29719293
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody (ab85936)IHC image of ab85936 staining in human breast ductal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85936, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-SNAIL + SLUG antibody (ab85936)All lanes : Anti-SNAIL + SLUG antibody (ab85936) at 1 µg/ml
Lane 1 : SLUG (SNAI2) Human - GST Tagged Recombinant Protein
Lane 2 : SNAIL (SNAI1) - GST Tagged Recombinant Protein
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Please note this antibody has only been shown to detect recombinant protein and we are unable to detect endogenous protein by western blot, in our lab. Please contact our scientific support services if you have any queries regarding this antibody.
