Immunohistochemical analysis of formalin-fixed and paraffin-embedded human cervical carcinoma tissue labeling SOX2 with ab218520 at 1 μg/mL dilution, followed by conjugation to the secondary antibody.

Anti-SOX2 antibody [SOX2/1791] (ab218520) at 2 µg/ml + A549 (human lung carcinoma cell line) whole cell lysate

Predicted band size: 34 kDa

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue labelling SOX2 with ab218520. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.

Immunohistochemical analysis of formalin-fixed and paraffin-embedded human lung carcinoma tissue labeling SOX2 with ab218520.

All lanes : Anti-SOX2 antibody [SOX2/1791] (ab218520)

Lane 1 : Recombinant SOX2 protein fragment
Lane 2 : NCCIT (human pluripotent embryonic carcinoma cell line) cell lysate

Predicted band size: 34 kDa

ab218520 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.