Anti-Ndufs4 antibody [2C7CD4AG3] (ab87399)
![Anti-Ndufs4 antibody [2C7CD4AG3] (ab87399)](https://www.abcam.com/ps/products/87/ab87399/Images/ab87399-102494-anti-ndufs4-antibody-2c7cd4ag3-western-blot.jpg "Anti-Ndufs4 antibody [2C7CD4AG3] (ab87399)")
Key features and details
- Mouse monoclonal [2C7CD4AG3] to Ndufs4
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
Overview
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Product name
Anti-Ndufs4 antibody [2C7CD4AG3]
See all Ndufs4 primary antibodies -
Description
Mouse monoclonal [2C7CD4AG3] to Ndufs4 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Recombinant full length protein. This information is considered to be commercially sensitive.
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: HEPES, Sodium chloride -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
2C7CD4AG3 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Ndufs4 antibody [2C7CD4AG3] (ab87399)All lanes : Anti-Ndufs4 antibody [2C7CD4AG3] (ab87399)
Lane 1 : Isolated mitochondria from human heart at 15 µg
Lane 2 : Isolated mitochondria from bovine heart at 6 µg
Lane 3 : Isolated mitochondria from rat heart at 30 µg
Lane 4 : Isolated mitochondria from mouse heart at 30 µg
Predicted band size: 20 kDa
Observed band size: 20 kDa
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![Flow Cytometry - Anti-Ndufs4 antibody [2C7CD4AG3] (ab87399)](https://www.abcam.com/ps/products/87/ab87399/Images/ab87399-102493-anti-ndufs4-antibody-2c7cd4ag3-flow-cytometry.jpg)
Flow Cytometry - Anti-Ndufs4 antibody [2C7CD4AG3] (ab87399)Overlay histogram showing HepG2 cells stained with ab87399 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab87399, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
