Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸ 10 µl 123 964 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3130] to SOX2
Suitable for: IHC-P, WB, IP
Reacts with: Human

Product name

Anti-SOX2 antibody [EPR3130]
See all SOX2 primary antibodies
Description

Rabbit monoclonal [EPR3130] to SOX2
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human SOX2 aa 250-350. The exact sequence is proprietary.
Positive control
- NCCIT cell lysate; Human fetal brain, Human normal brain and glioma tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3130
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3130] (ab133337)

Immunohistochemical analysis of Formalin-fixed, paraffin embedded Human fetal brain tissue labelling SOX2 with ab133337 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3130] (ab133337)

Immunohistochemical analysis of Formalin-fixed, paraffin embedded Human glioma tissue labelling SOX2 with ab133337 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3130] (ab133337)

Immunohistochemical analysis of Formalin-fixed, paraffin embedded Human brain tissue staining SOX2 with ab133337 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-SOX2 antibody [EPR3130] (ab133337)

Anti-SOX2 antibody [EPR3130] (ab133337) at 1/500 dilution + NCCIT cell lysate at 10 µg

Secondary
Goat Anti-rabbit HRP at 1/2000 dilution

Predicted band size: 34 kDa
Observed band size: 35 kDa
why is the actual band size different from the predicted?
Anti-SOX2 antibody [EPR3130] (ab133337)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com