Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human lungcarcinoma tissue labeling SOX2 with ab218521.

All lanes : Anti-SOX2 antibody [SOX2/1792] (ab218521)

Lane 1 : Recombinant SOX2 protein fragment
Lane 2 : NCCIT Cell Lysate

Predicted band size: 34 kDa

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse intestine tissue labeling SOX2 with ab218521 at 2 µg/mL (30 minutes at room temperature). Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 45 minutes at 95°C followed by cooling at room temperature for 20 minutes.

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human cervical carcinoma tissue labeling SOX2 with ab218521 at 1 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue labeling SOX2 with ab218521 at 2 µg/mL (30 minutes at room temperature). Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 45 minutes at 95°C followed by cooling at room temperature for 20 minutes.

ab218521 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.