Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hexokinase II antibody [3D3] (ab104836)

Key features and details

  • Mouse monoclonal [3D3] to Hexokinase II
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Hexokinase II antibody [3D3]
    See all Hexokinase II primary antibodies

  • Description

    Mouse monoclonal [3D3] to Hexokinase II

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human Hexokinase II.

  • Positive control

    • WB: Jurkat, HeLa and HEK293 cell lysates. IHC-P: Human esophagus and lung cancer tissues. Flow Cyt: K562 cells.

Properties

Images

  • Western blot - Anti-Hexokinase II antibody [3D3] (ab104836)
    Western blot - Anti-Hexokinase II antibody [3D3] (ab104836)
    All lanes : Anti-Hexokinase II antibody [3D3] (ab104836) at 1/500 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : HK2 knockout HEK-293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 102 kDa
    Observed band size: 102 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab104836 observed at 102 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.

    ab104836 was shown to react with Hexokinase II in wild-type HEK-293T cells in western blot. The band observed in HK2 knockout sample lane below 102 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and HK2 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab104836 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 500 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [3D3] (ab104836)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [3D3] (ab104836) This image is courtesy of an Abreview submitted by Ms Hadas Pahima

    Immunohistochemical analysis of human skin tissue, staining Hexokinase II with ab104836.

    Tissue was fixed with paraformaldehyde and blocked with 10% BSA for 2 hours at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/250 in 5% NGS + 10% BSA in PBS) for 16 hours at 4°C. An HRP-conjugated goat anti-mouse polyclonal IgG (1/250) was used as the secondary antibody.

    See Abreview

  • Flow Cytometry - Anti-Hexokinase II antibody [3D3] (ab104836)
    Flow Cytometry - Anti-Hexokinase II antibody [3D3] (ab104836)
    ab104836 staining Hexokinase II in K562 cells by Immunofluorescence (green) and negative control (purple).
  • Western blot - Anti-Hexokinase II antibody [3D3] (ab104836)
    Western blot - Anti-Hexokinase II antibody [3D3] (ab104836)
    All lanes : Anti-Hexokinase II antibody [3D3] (ab104836) at 1/500 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HEK293 cell lysate

    Predicted band size: 102 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [3D3] (ab104836)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [3D3] (ab104836)

    ab104836 at 1/200 dilution staining Hexokinase II in human esophagus cancer by Immunohistochemistry, Paraffin-embedded tissue with DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [3D3] (ab104836)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [3D3] (ab104836)

    ab104836 at 1/200 dilution staining Hexokinase II in human lung cancer by Immunohistochemistry, Paraffin-embedded tissue with DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Hexokinase II antibody [3D3] (ab104836)

© 2021 Astana Biomed Group LLP. All rights reserved.