Anti-Hexokinase II antibody [3D3] (ab104836)
![Anti-Hexokinase II antibody [3D3] (ab104836)](https://www.abcam.com/ps/products/104/ab104836/Images/ab104836-448165-anti-hexokinase-ii-antibody-3d3-western-blot-hek293-wt-hek293-hk2-knockout-hela-a549.jpg "Anti-Hexokinase II antibody [3D3] (ab104836)")
Key features and details
- Mouse monoclonal [3D3] to Hexokinase II
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Hexokinase II antibody [3D3]
See all Hexokinase II primary antibodies -
Description
Mouse monoclonal [3D3] to Hexokinase II -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human Hexokinase II.
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Positive control
- WB: Jurkat, HeLa and HEK293 cell lysates. IHC-P: Human esophagus and lung cancer tissues. Flow Cyt: K562 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3D3 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Hexokinase II antibody [3D3] (ab104836)All lanes : Anti-Hexokinase II antibody [3D3] (ab104836) at 1/500 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : HK2 knockout HEK-293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 102 kDaLanes 1 - 4: Merged signal (red and green). Green - ab104836 observed at 102 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.
ab104836 was shown to react with Hexokinase II in wild-type HEK-293T cells in western blot. The band observed in HK2 knockout sample lane below 102 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and HK2 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab104836 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 500 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [3D3] (ab104836)](https://www.abcam.com/ps/products/104/ab104836/Images/ab104836-117438-anti-hexokinase-ii-antibody-3d3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [3D3] (ab104836) This image is courtesy of an Abreview submitted by Ms Hadas PahimaImmunohistochemical analysis of human skin tissue, staining Hexokinase II with ab104836.
Tissue was fixed with paraformaldehyde and blocked with 10% BSA for 2 hours at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/250 in 5% NGS + 10% BSA in PBS) for 16 hours at 4°C. An HRP-conjugated goat anti-mouse polyclonal IgG (1/250) was used as the secondary antibody. -
![Flow Cytometry - Anti-Hexokinase II antibody [3D3] (ab104836)](https://www.abcam.com/ps/products/104/ab104836/Images/ab104836-117439-anti-hexokinase-ii-antibody-3d3-flow-cytometry.jpg)
Flow Cytometry - Anti-Hexokinase II antibody [3D3] (ab104836)ab104836 staining Hexokinase II in K562 cells by Immunofluorescence (green) and negative control (purple). -
![Western blot - Anti-Hexokinase II antibody [3D3] (ab104836)](https://www.abcam.com/ps/products/104/ab104836/Images/ab104836-117442-anti-hexokinase-ii-antibody-3d3-western-blot.jpg)
Western blot - Anti-Hexokinase II antibody [3D3] (ab104836)All lanes : Anti-Hexokinase II antibody [3D3] (ab104836) at 1/500 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HEK293 cell lysate
Predicted band size: 102 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [3D3] (ab104836)](https://www.abcam.com/ps/products/104/ab104836/Images/ab104836-117441-anti-hexokinase-ii-antibody-3d3-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [3D3] (ab104836)ab104836 at 1/200 dilution staining Hexokinase II in human esophagus cancer by Immunohistochemistry, Paraffin-embedded tissue with DAB staining.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [3D3] (ab104836)](https://www.abcam.com/ps/products/104/ab104836/Images/ab104836-117440-anti-hexokinase-ii-antibody-3d3-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [3D3] (ab104836)ab104836 at 1/200 dilution staining Hexokinase II in human lung cancer by Immunohistochemistry, Paraffin-embedded tissue with DAB staining.
