Anti-Cyp3a1 antibody [P6] (ab22724)
![Anti-Cyp3a1 antibody [P6] (ab22724)](https://www.abcam.com/ps/products/22/ab22724/Images/ab22724-32527-anti-cytochrome-p450-3a1-antibody-p6-flow-cytometry.jpg "Anti-Cyp3a1 antibody [P6] (ab22724)")
Key features and details
- Mouse monoclonal [P6] to Cyp3a1
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Cyp3a1 antibody [P6]
See all Cyp3a1 primary antibodies -
Description
Mouse monoclonal [P6] to Cyp3a1 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Rat Cyp3a1.
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General notes
This product was previously labelled as Cytochrome P450 3A1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
P6 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-Cyp3a1 antibody [P6] (ab22724)
Overlay histogram showing HepG2 cells stained with ab22724 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22724, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.