Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸ 10 µl 137 366 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ADH5 antibody [EPR12885(B)] (ab177932)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12885(B)] to ADH5
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-ADH5 antibody [EPR12885(B)]
See all ADH5 primary antibodies
Description

Rabbit monoclonal [EPR12885(B)] to ADH5
Host species

Rabbit
Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Tested Applications & Species

Application	Species
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human ADH5 aa 1-100. The exact sequence is proprietary.
Database link: P11766
Positive control
- WB: Human liver, Mouse liver, Human fetal liver, HepG2, Human fetal kidney, SH-SY5Y, Mouse brain, Rat liver, and Rat brain lysates. IHC-P: human kidney tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12885(B)
Isotype

IgG
Research areas

Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)

Anti-ADH5 antibody [EPR12885(B)] (ab177932) at 1/5000 dilution (Purified) + Human liver lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 40 kDa
Observed band size: 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling ADH5 with purified ab177932 at 1/8000 dilution (0.06 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)

All lanes : Anti-ADH5 antibody [EPR12885(B)] (ab177932) at 1/5000 dilution (Purified)

Lane 1 : Mouse liver lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat liver lysates
Lane 4 : Rat brain lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 40 kDa
Observed band size: 40 kDa

ADH5 is highly expressed in liver, kidney and low expressed in brain as is described in PMID: 26412304
Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)

All lanes : Anti-ADH5 antibody [EPR12885(B)] (ab177932) at 1/1000 dilution ((unpurified))

Lane 1 : Human fetal liver lysates
Lane 2 : HepG2 lysates
Lane 3 : Human fetal kidney lysates
Lane 4 : SH-SY5Y lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ADH5 with unpurified ab177932 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ADH5 with unpurified ab177932 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-ADH5 antibody [EPR12885(B)] (ab177932)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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