Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

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Anti-ADH5 antibody [EPR12886(B)] - C-terminal (ab174283)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12886(B)] to ADH5 - C-terminal
Suitable for: Flow Cyt, IHC-P, WB
Reacts with: Human

Product name

Anti-ADH5 antibody [EPR12886(B)] - C-terminal
See all ADH5 primary antibodies
Description

Rabbit monoclonal [EPR12886(B)] to ADH5 - C-terminal
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ADH5 aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P11766
Positive control
- Human fetal liver, Human fetal kidney, K562 and HepG2 lysates; Human heart and liver tissue; K562 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12886(B)
Isotype

IgG
Research areas

Western blot - Anti-ADH5 antibody [EPR12886(B)] - C-terminal (ab174283)

All lanes : Anti-ADH5 antibody [EPR12886(B)] - C-terminal (ab174283) at 1/1000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : K562 cell line lysate
Lane 4 : HepG2 lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/500 dilution

Developed using the ECL technique.

Predicted band size: 40 kDa
Flow Cytometry - Anti-ADH5 antibody [EPR12886(B)] - C-terminal (ab174283)

Flow cytometric analysis of permeabilized K562 cells labeling ADH5 with ab174283 at 1/50 (red) or a rabbit IgG (negative) (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12886(B)] - C-terminal (ab174283)

Immunohistochemical analysis of paraffin embedded Human liver tissue labeling ADH5 with ab174283 at 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12886(B)] - C-terminal (ab174283)

Immunohistochemical analysis of paraffin embedded Human heart tissue labeling ADH5 with ab174283 at 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-ADH5 antibody [EPR12886(B)] - C-terminal (ab174283)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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