Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4439] to Alcohol Dehydrogenase
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Alcohol Dehydrogenase antibody [EPR4439]
    See all Alcohol Dehydrogenase primary antibodies

  • Description

    Rabbit monoclonal [EPR4439] to Alcohol Dehydrogenase

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: lysate from HepG2 cells, Human fetal, mouse or rat liver; IHC-P: Human liver tissue; ICC/IF: HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
    Western blot - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
    All lanes : Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Mouse liver lysate
    Lane 3 : Human fetal liver lysate
    Lane 4 : Rat liver lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 40 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
    Immunocytochemistry/ Immunofluorescence - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203) This image is courtesy of an Abreview submitted by Armen Petrosyan

    ab108203 staining Alcohol Dehydrogenase in the HepG2 cell line from Human liver by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% Serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/50) for 1 hour at 22°C. An Alexa Fluor®594-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody. Nucleus was stained with DAPI (blue).

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)

    ab108203 at 1/250 dilution staining Alcohol Dehydrogenase in paraffin-embedded Human liver tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
    Immunocytochemistry/ Immunofluorescence - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
    ab108203 at 1/500 dilution staining Alcohol Dehydrogenase in HepG2 cells by Immunofluorescence.
  • Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
    Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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