Anti-Gankyrin antibody [OTI3F6] (ab118009)
![Anti-Gankyrin antibody [OTI3F6] (ab118009)](https://www.abcam.com/ps/products/118/ab118009/Images/Proteasome-26S-S10-Primary-antibodies-ab118009-15.jpg "Anti-Gankyrin antibody [OTI3F6] (ab118009)")
Key features and details
- Mouse monoclonal [OTI3F6] to Gankyrin
- Suitable for: WB, Flow Cyt
- Reacts with: Dog, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-Gankyrin antibody [OTI3F6]
See all Gankyrin primary antibodies -
Description
Mouse monoclonal [OTI3F6] to Gankyrin -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Dog, Human, African green monkey -
Immunogen
Recombinant full length protein corresponding to Human Gankyrin aa 1 to the C-terminus. Produced in HEK-293T cells (NP_002805).
Database link: O75832 -
Positive control
- WB: HEK-293T cell lysate transfected with the pCMV6-ENTRY Gankyrin cDNA; HepG2 cell extract. Flow Cyt: HeLa and Jurkat cells.
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General notes
Clone OTI3F6 (formerly 3F6).
Previously labelled as Proteasome 26S S10.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol, PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI3F6 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Gankyrin antibody [OTI3F6] (ab118009)All lanes : Anti-Gankyrin antibody [OTI3F6] (ab118009) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs
Lane 2 : HEK-293T lysate transfected with pCMV6-ENTRY Proteasome 26S S10 cDNA for 48 hrs
Lysates/proteins at 5 µg per lane.
Predicted band size: 24 kDa
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![Western blot - Anti-Gankyrin antibody [OTI3F6] (ab118009)](https://www.abcam.com/ps/products/118/ab118009/Images/Proteasome-26S-S10-Primary-antibodies-ab118009-16.jpg)
Western blot - Anti-Gankyrin antibody [OTI3F6] (ab118009)All lanes : Anti-Gankyrin antibody [OTI3F6] (ab118009) at 1/2000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : HT-29 (human colorectal adenocarcinoma cell line) cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (canine kidney cell line) extract
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 24 kDa
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![Flow Cytometry - Anti-Gankyrin antibody [OTI3F6] (ab118009)](https://www.abcam.com/ps/products/118/ab118009/Images/Proteasome-26S-S10-Primary-antibodies-ab118009-17.jpg)
Flow Cytometry - Anti-Gankyrin antibody [OTI3F6] (ab118009)Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Gankyrin (red) using ab118009 at 1/100 dilution, compared to a nonspecific negative control antibody (blue).
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![Flow Cytometry - Anti-Gankyrin antibody [OTI3F6] (ab118009)](https://www.abcam.com/ps/products/118/ab118009/Images/Proteasome-26S-S10-Primary-antibodies-ab118009-18.jpg)
Flow Cytometry - Anti-Gankyrin antibody [OTI3F6] (ab118009)Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling Gankyrin (red) using ab118009 at 1/100 dilution, compared to a nonspecific negative control antibody (blue).
