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Anti-Gankyrin antibody [EPR14455] (ab182576)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14455] to Gankyrin
  • Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Gankyrin antibody [EPR14455]
    See all Gankyrin primary antibodies

  • Description

    Rabbit monoclonal [EPR14455] to Gankyrin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, HepG2, K562 and 293 cell lysate; Human infiltrating duct carcinoma of breast and lung adenocarcinoma tissues; 293 cells; K562 cells.

  • General notes

     

     

    Previously labelled as Proteasome 26S S10.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Gankyrin antibody [EPR14455] (ab182576)
    Western blot - Anti-Gankyrin antibody [EPR14455] (ab182576)
    All lanes : Anti-Gankyrin antibody [EPR14455] (ab182576) at 1/1000 dilution

    Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : PSMD10 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 24 kDa
    Observed band size: 24 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab182576 observed at 24 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab182576 Anti-Gankyrin antibody [EPR14455] was shown to specifically react with Gankyrin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265680 (knockout cell lysate ab258146) was used. Wild-type and Gankyrin knockout samples were subjected to SDS-PAGE. ab182576 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gankyrin antibody [EPR14455] (ab182576)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gankyrin antibody [EPR14455] (ab182576)

    Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling Gankyrin using ab182576 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG was used as secondary. Counterstain: Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-Gankyrin antibody [EPR14455] (ab182576)
    Western blot - Anti-Gankyrin antibody [EPR14455] (ab182576)
    All lanes : Anti-Gankyrin antibody [EPR14455] (ab182576) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gankyrin antibody [EPR14455] (ab182576)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gankyrin antibody [EPR14455] (ab182576)

    Immunohistochemical analysis of paraffin-embedded human infiltrating duct carcinoma of breast tissue labeling Gankyrin using ab182576 at 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as secondary.

    Counterstain: Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Gankyrin antibody [EPR14455] (ab182576)
    Immunocytochemistry/ Immunofluorescence - Anti-Gankyrin antibody [EPR14455] (ab182576)

    Immunofluorescence analysis of 293 cells labeling Gankyrin using ab182576 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde.

  • Flow Cytometry - Anti-Gankyrin antibody [EPR14455] (ab182576)
    Flow Cytometry - Anti-Gankyrin antibody [EPR14455] (ab182576)

    Flow cytometry analysis of K562 cells labeling Gankyrin using ab182576 at 1/80 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.

  • Anti-Gankyrin antibody [EPR14455] (ab182576)
    Anti-Gankyrin antibody [EPR14455] (ab182576)

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