Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 130 665 ₸

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Anti-Gankyrin antibody [EPR14455] (ab182576)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14455] to Gankyrin
Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
Knockout validated
Reacts with: Human

Product name

Anti-Gankyrin antibody [EPR14455]
See all Gankyrin primary antibodies
Description

Rabbit monoclonal [EPR14455] to Gankyrin
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa, HepG2, K562 and 293 cell lysate; Human infiltrating duct carcinoma of breast and lung adenocarcinoma tissues; 293 cells; K562 cells.
General notes
Previously labelled as Proteasome 26S S10.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14455
Isotype

IgG
Research areas

Western blot - Anti-Gankyrin antibody [EPR14455] (ab182576)

All lanes : Anti-Gankyrin antibody [EPR14455] (ab182576) at 1/1000 dilution

Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : PSMD10 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 24 kDa
Observed band size: 24 kDa

Lanes 1-4: Merged signal (red and green). Green - ab182576 observed at 24 kDa. Red - loading control ab8245 observed at 36 kDa.

ab182576 Anti-Gankyrin antibody [EPR14455] was shown to specifically react with Gankyrin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265680 (knockout cell lysate ab258146) was used. Wild-type and Gankyrin knockout samples were subjected to SDS-PAGE. ab182576 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gankyrin antibody [EPR14455] (ab182576)

Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling Gankyrin using ab182576 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG was used as secondary. Counterstain: Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-Gankyrin antibody [EPR14455] (ab182576)

All lanes : Anti-Gankyrin antibody [EPR14455] (ab182576) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : 293 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gankyrin antibody [EPR14455] (ab182576)

Immunohistochemical analysis of paraffin-embedded human infiltrating duct carcinoma of breast tissue labeling Gankyrin using ab182576 at 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as secondary.

Counterstain: Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Gankyrin antibody [EPR14455] (ab182576)

Immunofluorescence analysis of 293 cells labeling Gankyrin using ab182576 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde.
Flow Cytometry - Anti-Gankyrin antibody [EPR14455] (ab182576)

Flow cytometry analysis of K562 cells labeling Gankyrin using ab182576 at 1/80 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.
Anti-Gankyrin antibody [EPR14455] (ab182576)

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