Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AKT1 (phospho S473) antibody [AktS473-B9] (ab278559)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [AktS473-B9] to AKT1 (phospho S473)
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-AKT1 (phospho S473) antibody [AktS473-B9]
    See all AKT1 primary antibodies

  • Description

    Rabbit monoclonal [AktS473-B9] to AKT1 (phospho S473)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human AKT1 (phospho S473). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P31749

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: NIH/3T3 (treated with PDGF) cell extract. Flow cyt: Jurkat cells (treated with 20% FBS); C2C12 cells (treated with staurosporine).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Flow Cytometry - Anti-AKT1 (phospho S473) antibody [AktS473-B9] (ab278559)
    Flow Cytometry - Anti-AKT1 (phospho S473) antibody [AktS473-B9] (ab278559)
    Flow cytometric analysis of Jurkat cells treated with LY294002 (red) or with 20% FBS (green) using ab278559 at 0.05 µg/mL.
  • Western blot - Anti-AKT1 (phospho S473) antibody [AktS473-B9] (ab278559)
    Western blot - Anti-AKT1 (phospho S473) antibody [AktS473-B9] (ab278559)
    All lanes : Anti-AKT1 (phospho S473) antibody [AktS473-B9] (ab278559) at 0.0005 µg/ml

    Lane 1 : NIH3T3 cells treated with PDGF
    Lane 2 : NIH3T3 cells treated without PDGF

    Predicted band size: 55 kDa
    Observed band size: 58 kDa
    why is the actual band size different from the predicted?

  • Flow Cytometry - Anti-AKT1 (phospho S473) antibody [AktS473-B9] (ab278559)
    Flow Cytometry - Anti-AKT1 (phospho S473) antibody [AktS473-B9] (ab278559)
    Flow cytometric analysis of C2C12 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control (orange) or untreated (red) or treated with staurosporine (green) using ab278559 at 0.1 µg/mL.
  • Anti-AKT1 (phospho S473) antibody [AktS473-B9] (ab278559)
    Anti-AKT1 (phospho S473) antibody [AktS473-B9] (ab278559)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.