Antibodies, Proteins, Kits and Reagents for Life Science

338 390 ₸ Product size

100 µl 338 390 ₸

Order now and get it on Tuesday March 09, 2021

Anti-FKBP38 antibody [EPR7441(2)] (ab129113)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7441(2)] to FKBP38
Suitable for: WB, Flow Cyt, ICC/IF
Knockout validated
Reacts with: Human

Product name

Anti-FKBP38 antibody [EPR7441(2)]
See all FKBP38 primary antibodies
Description

Rabbit monoclonal [EPR7441(2)] to FKBP38
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human FKBP38 (N terminal). The exact sequence is proprietary.
Positive control
- HeLa cells; 293T, HeLa and Jurkat whole cell lysate (ab7899).
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR7441(2)
Isotype

IgG
Research areas

Western blot - Anti-FKBP38 antibody [EPR7441(2)] (ab129113)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: ProteinX knockout HAP1 cell lysate (20 µg)
Lane 3: CellX1 cell lysate (20 µg)
Lane 4: CellX2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab129113 observed at 51 kDa. Red - loading control, ab18058, observed at 124 kDa.

ab129113 was shown to specifically react with FKBP38 when FKBP38 knockout samples were used. Wild-type and FKBP38 knockout samples were subjected to SDS-PAGE. ab129113 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-FKBP38 antibody [EPR7441(2)] (ab129113)

All lanes : Anti-FKBP38 antibody [EPR7441(2)] (ab129113) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 45 kDa
Immunocytochemistry/ Immunofluorescence - Anti-FKBP38 antibody [EPR7441(2)] (ab129113)

ab129113, at 1/100 dilution, staining FKBP38 in HeLa cells by Immunofluorescence.
Anti-FKBP38 antibody [EPR7441(2)] (ab129113)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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