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Anti-Gankyrin antibody [EPR14456(2)] (ab188315)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14456(2)] to Gankyrin
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Gankyrin antibody [EPR14456(2)]
    See all Gankyrin primary antibodies

  • Description

    Rabbit monoclonal [EPR14456(2)] to Gankyrin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Hela, HepG2, 293, C6, Raw264.7 and K562 cell lysates. HeLa cells.

  • General notes

     

     

    Previously labelled as Proteasome 26S S10.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Gankyrin antibody [EPR14456(2)] (ab188315)
    Western blot - Anti-Gankyrin antibody [EPR14456(2)] (ab188315)
    All lanes : Anti-Gankyrin antibody [EPR14456(2)] (ab188315) at 1/1000 dilution

    Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : PSMD10 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 24 kDa
    Observed band size: 24 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab188315 observed at 24 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab188315 Anti-Gankyrin antibody [EPR14456(2)] was shown to specifically react with Gankyrin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265680 (knockout cell lysate ab258146) was used. Wild-type and Gankyrin knockout samples were subjected to SDS-PAGE. ab188315 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-Gankyrin antibody [EPR14456(2)] (ab188315)
    Flow Cytometry - Anti-Gankyrin antibody [EPR14456(2)] (ab188315)

    Flow cytometric analysis of HeLa cells fixed in 2% paraformaldehyde labeling Gankyrin with ab188315 at 1/110 dilution and Goat anti-rabbit IgG (FITC) at 1/150 dilution.  Rabbit monoclonal IgG was used as an isotype control.

  • Western blot - Anti-Gankyrin antibody [EPR14456(2)] (ab188315)
    Western blot - Anti-Gankyrin antibody [EPR14456(2)] (ab188315)
    All lanes : Anti-Gankyrin antibody [EPR14456(2)] (ab188315) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw 264.7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa

  • Western blot - Anti-Gankyrin antibody [EPR14456(2)] (ab188315)
    Western blot - Anti-Gankyrin antibody [EPR14456(2)] (ab188315)
    All lanes : Anti-Gankyrin antibody [EPR14456(2)] (ab188315) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa

  • Western blot - Anti-Gankyrin antibody [EPR14456(2)] (ab188315)
    Western blot - Anti-Gankyrin antibody [EPR14456(2)] (ab188315)
    All lanes : Anti-Gankyrin antibody [EPR14456(2)] (ab188315) at 1/10000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Hela cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa

  • Anti-Gankyrin antibody [EPR14456(2)] (ab188315)
    Anti-Gankyrin antibody [EPR14456(2)] (ab188315)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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