Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ABCB11/BSEP antibody [EPR22670-64] (ab255605)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22670-64] to ABCB11/BSEP
Suitable for: ICC, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-ABCB11/BSEP antibody [EPR22670-64]
See all ABCB11/BSEP primary antibodies
Description

Rabbit monoclonal [EPR22670-64] to ABCB11/BSEP
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC	Human
IHC-P	Human Mouse Rat

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human hepatocellular carcinoma and liver tissue. ICC: HEK-293T cells transfected with myc-tagged BSEP. Flow Cyt: HEK-293T cells transfected with myc-tagged BSEP.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22670-64
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] (ab255605)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling BSEP with ab255605 at 1/500 dilution (1.288µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in canaliculi of human liver (PMID: 26182153) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] (ab255605)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling BSEP with ab255605 at 1/ 500 dilution (1.288µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in canaliculi of human hepatocellular carcinoma (PMID: 26735860) is observed.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] (ab255605)

Immunohistochemical analysis of paraffin-embedded human cholangiocarcinomas tissue labeling BSEP with ab255605 at 1/500 dilution (1.288µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Negative staining in human cholangiocarcinomas (PMID: 26735860) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Immunocytochemistry - Anti-ABCB11/BSEP antibody [EPR22670-64] (ab255605)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labeling BSEP with ab255605 at 1/100, (6.4µg/ml) dilution, followed by aab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000, (2µg/ml) dilution (Red). Confocal image showing membranous and cytoplasmic staining in 293T cells transfected with myc-tagged BSEP expression vector. Myc-Tag Mouse mAb (Alexa Fluor® 488) was used as a counterstain (Green) at 1/100 dilution (Red). The nuclear counterstain was DAPI (Blue).

Negative control: myc-transfected cells.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^® 488 Goat anti-Rabbit secondary at 1/1000, 2µg/ml dilution.
Flow Cytometry - Anti-ABCB11/BSEP antibody [EPR22670-64] (ab255605)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HEK-293T (human embryonic kidney epithelial cell) transfected with myc-tagged BSEP expression vector cells labeling BSEP with ab255605 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on myc(+) population.
Anti-ABCB11/BSEP antibody [EPR22670-64] (ab255605)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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