Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (ab256536)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22670-64] to ABCB11/BSEP - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free
    See all ABCB11/BSEP primary antibodies

  • Description

    Rabbit monoclonal [EPR22670-64] to ABCB11/BSEP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, ICC/IFmore details
    Unsuitable for: IP or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human hepatocellular carcinoma and liver tissue. ICC/IF: HEK-293T cells transfected with myc-tagged BSEP. Flow Cyt (intra): HEK-293T cells transfected with myc-tagged BSEP.

  • General notes

    ab256536 is the carrier-free version of ab255605.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (ab256536)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (ab256536)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling BSEP with ab255605 at 1/500 dilution (1.288µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in canaliculi of human liver (PMID: 26182153) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255605).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (ab256536)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (ab256536)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling BSEP with ab255605 at 1/ 500 dilution (1.288µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in canaliculi of human hepatocellular carcinoma (PMID: 26735860) is observed.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255605).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (ab256536)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (ab256536)

    Immunohistochemical analysis of paraffin-embedded human cholangiocarcinomas tissue labeling BSEP with ab255605 at 1/500 dilution (1.288µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Negative staining in human cholangiocarcinomas (PMID: 26735860) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255605).

  • Flow Cytometry - Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (ab256536)
    Flow Cytometry - Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (ab256536)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HEK-293T (human embryonic kidney epithelial cell) transfected with myc-tagged BSEP expression vector cells labeling BSEP with ab255605 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on myc(+) population.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255605).

  • Immunocytochemistry/ Immunofluorescence - Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (ab256536)
    Immunocytochemistry/ Immunofluorescence - Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (ab256536)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labeling BSEP with ab255605 at 1/100, (6.4µg/ml) dilution, followed by ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary antibody at 1/1000, (2µg/ml) dilution (Green). Confocal image showing membranous and cytoplasmic staining in 293T cells transfected with myc-tagged BSEP expression vector. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Negative control: myc-transfected cells.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000, 2µg/ml dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255605).

  • Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (ab256536)
    Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (ab256536)

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