Anti-Gankyrin antibody [EPR14455] - BSA and Azide free (ab238999)
![Anti-Gankyrin antibody [EPR14455] - BSA and Azide free (ab238999)](https://www.abcam.com/ps/products/238/ab238999/Images/ab238999-412696-anti-gankyrin-antibody-epr14455-western-blot-human-knockout-lysate.jpg "Anti-Gankyrin antibody [EPR14455] - BSA and Azide free (ab238999)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14455] to Gankyrin - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Gankyrin antibody [EPR14455] - BSA and Azide free
See all Gankyrin primary antibodies -
Description
Rabbit monoclonal [EPR14455] to Gankyrin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, HepG2, K562 and 293 cell lysate; Human infiltrating duct carcinoma of breast and lung adenocarcinoma tissues; 293 cells; K562 cells.
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General notes
ab238999 is the carrier-free version of ab182576 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab238999 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Previously labelled as Proteasome 26S S10.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14455 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Gankyrin antibody [EPR14455] - BSA and Azide free (ab238999)All lanes : Anti-Gankyrin antibody [EPR14455] (ab182576) at 1/1000 dilution
Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : PSMD10 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDaThis data was developed using ab182576, the same antibody clone in a different buffer formulation.
Lanes 1-4: Merged signal (red and green). Green - ab182576 observed at 24 kDa. Red - loading control ab8245 observed at 36 kDa.
ab182576 Anti-Gankyrin antibody [EPR14455] was shown to specifically react with Gankyrin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265680 (knockout cell lysate ab258146) was used. Wild-type and Gankyrin knockout samples were subjected to SDS-PAGE. ab182576 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gankyrin antibody [EPR14455] - BSA and Azide free (ab238999)](https://www.abcam.com/ps/products/238/ab238999/Images/ab238999-4-ab182576213876ab182576ihc2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gankyrin antibody [EPR14455] - BSA and Azide free (ab238999)Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling Gankyrin using ab182576 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG was used as secondary. Counterstain: Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182576).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-Gankyrin antibody [EPR14455] - BSA and Azide free (ab238999)](https://www.abcam.com/ps/products/238/ab238999/Images/ab238999-2-ab182576213871ab182576fc.jpg)
Flow Cytometry - Anti-Gankyrin antibody [EPR14455] - BSA and Azide free (ab238999)Flow cytometry analysis of K562 cells labeling Gankyrin using ab182576 at 1/80 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182576).
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![Immunocytochemistry/ Immunofluorescence - Anti-Gankyrin antibody [EPR14455] - BSA and Azide free (ab238999)](https://www.abcam.com/ps/products/238/ab238999/Images/ab238999-3-ab182576213873ab182576if.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Gankyrin antibody [EPR14455] - BSA and Azide free (ab238999)Immunofluorescence analysis of 293 cells labeling Gankyrin using ab182576 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182576).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gankyrin antibody [EPR14455] - BSA and Azide free (ab238999)](https://www.abcam.com/ps/products/238/ab238999/Images/ab238999-317681-2389991.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gankyrin antibody [EPR14455] - BSA and Azide free (ab238999)Immunohistochemical analysis of paraffin-embedded human infiltrating duct carcinoma of breast tissue labeling Gankyrin using ab182576 at 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as secondary.
Counterstain: Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab182576).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Anti-Gankyrin antibody [EPR14455] - BSA and Azide free (ab238999)](https://www.abcam.com/ps/products/238/ab238999/Images/ab238999-6-benefits-of-recombinant-antibodies.png)
Anti-Gankyrin antibody [EPR14455] - BSA and Azide free (ab238999)
