Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] - BSA and Azide free (ab249366)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8826(2)(B)] to Proteasome subunit beta type 2/PSMB2 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] - BSA and Azide free
    See all Proteasome subunit beta type 2/PSMB2 primary antibodies

  • Description

    Rabbit monoclonal [EPR8826(2)(B)] to Proteasome subunit beta type 2/PSMB2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab249366 is the carrier-free version of ab166628 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab249366 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as PSMB2, Proteasome subunit beta type 2

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR8826(2)(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] - BSA and Azide free (ab249366)
    Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] - BSA and Azide free (ab249366)
    All lanes : Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : LoVo cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 23 kDa



    This data was developed using ab166628, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] - BSA and Azide free (ab249366)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] - BSA and Azide free (ab249366)
    This data was developed using ab166628, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human liver tissue labeling Proteasome subunit beta type 2/PSMB2 with ab166628 at a 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] - BSA and Azide free (ab249366)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] - BSA and Azide free (ab249366)
    This data was developed using ab166628, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling Proteasome subunit beta type 2/PSMB2 with ab166628 at a 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] - BSA and Azide free (ab249366)
    Flow Cytometry - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] - BSA and Azide free (ab249366)
    This data was developed using ab166628, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized Jurkat cells using ab166628 at a 1/100 dilution (red) or a Rabbit IgG (negative) (green).
  • Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] - BSA and Azide free (ab249366)
    Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] - BSA and Azide free (ab249366)
    Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628) at 1/1000 dilution + immunoprecipitation pellet from HeLa cell lysate at 10 µg

    Secondary
    HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.

    Predicted band size: 23 kDa



    This data was developed using ab166628, the same antibody clone in a different buffer formulation.

     

  • Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] - BSA and Azide free (ab249366)
    Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] - BSA and Azide free (ab249366)

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